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- Yabuki Yasushi
- Dept. Geno. Neurol., IMEG., Kumamoto Univ.
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- Maeda Kohei
- Dept. Geno. Neurol., IMEG., Kumamoto Univ.
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- Mizobata Tomohiro
- Dept. Chem. & Biotech., Grad. Sch. Eng., Tottori Univ.
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- Kawata Yasushi
- Dept. Chem. & Biotech., Grad. Sch. Eng., Tottori Univ.
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- Asamitsu Sefan
- Dept. Geno. Neurol., IMEG., Kumamoto Univ.
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- Shioda Norifumi
- Dept. Geno. Neurol., IMEG., Kumamoto Univ.
Bibliographic Information
- Other Title
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- RNAグアニン四重鎖はα-シヌクレインの液-固相転移を促進する
Abstract
<p>Aggregation of α-synuclein (α-Syn) triggers development of α-synucleinopathies such as Parkinson’s disease and Dementia with Lewy Bodies. It also has known that α-Syn can spread in the whole brain, like as prions. However, the molecular mechanisms remain unclear. Here, we show that RNA G-quadruplexes (G4RNAs) promote α-Syn aggregation through liquid-liquid phase separation (LLPS). Electrophoretic mobility shift assay revealed that α-Syn significantly and preferentially formed a complex with G4RNAs than with mutated G4RNAs that is unable to form G4 and other structural RNAs (hairpin and polyA). α-Syn undergoes LLPS, and G4RNAs significantly facilitate liquid-to-solid transition of α-Syn under the condition of molecular crowding (15% polyethylene glycol). These observations suggest that α-Syn may undergoes phase transition by G4RNAs under the pathological condition in vivo. We would like to also discuss the role of G4RNA in α-Syn propagation in the meeting.</p>
Journal
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- Proceedings for Annual Meeting of The Japanese Pharmacological Society
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Proceedings for Annual Meeting of The Japanese Pharmacological Society 94 (0), 2-O-B2-4-, 2021
Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390287441187136256
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- NII Article ID
- 130008001439
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- ISSN
- 24354953
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed