Development of a novel treatment for interstitialcystitis using filtered adipose-derived stem cells lysate

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  • Shibayama Maaya
    Dept. Hospital Pharm., Grad. Sch. Pharm. Sci.,Nagoya City Univ.
  • Hotta Yuji
    Dept. Hospital Pharm., Grad. Sch. Pharm. Sci.,Nagoya City Univ.
  • Maeda Kotomi
    Dept. Hospital Pharm., Grad. Sch. Pharm. Sci.,Nagoya City Univ.
  • Matsumoto Seiji
    Cent. Adv. Res. Edu., Asahikawa Med.Univ.
  • Kataoka Tomoya
    Dept. Clinical Pharm., Grad. Sch. Med. Sci.,Nagoya City Univ.
  • Yamamoto Tokunori
    Dept. Neph., Grad. Sch. Med. Sci., NagoyaUniv.
  • Kimura Kazunori
    Dept. Hospital Pharm., Grad. Sch. Pharm. Sci.,Nagoya City Univ. Dept. Clinical Pharm., Grad. Sch. Med. Sci.,Nagoya City Univ.

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Other Title
  • 脂肪由来幹細胞濾液を用いた間質性膀胱炎の新たな治療法の開発

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<p>Hypothesis Interstitial cystitis (IC) is a chronic inflammatorydisease characterized by pain in the bladder and voiding symptoms. To date,there are no effective treatments for IC. We investigated whether filteredadipose-derived stem cell lysate (FADSCL) alleviates IC in a rat model ofHCl-induced cystitis.</p><p>Materials and methods Rat adipose-derived stem cells were collectedfrom the subcutaneous fat of rats, and FADSCL (lysate of 1×107 cells/PBS) wasprepared. Female Fisher 344 rats, aged eight weeks, were divided into thesham+PBS (sham, n=10), HCl+PBS (HCl, n=12), and HCl+FADSCL (FADSCL, n=9) groups.Saline or 0.1 M HCl (250μl/body) was intravesically administered from atransurethral catheter. Bladder function was investigated after one week bycystometrography. A sample of bladder tissue was used for RNA-seq analysis.</p><p>Results The intercontraction intervals (ICIs) in the HCl group weresignificantly shorter than those in the sham group (P<0.01), while the ICIsof the FADSCL group were significantly longer than those of the HCl group(P<0.01). There was no significant difference between the groups with respectto the maximum voiding pressure and bladder weight. RNA-Seq analysis revealed anincrease in immune and inflammatory signaling in the HCl group compared to thatin the sham group, and these signals were suppressed in the FADSCL group.</p><p>Conclusion FADSCL might alleviate IC via anti-inflammatory actionsand suppress the immune response. Thus, FADSCL could serve as a noveltherapeutic agent for IC.</p>

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