Two aquaporins, SIP1;1 and PIP1;2, mediate water transport for pollen hydration in the <i>Arabidopsis</i> pistil
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- Windari Endang Ayu
- Graduate School of Bioresources, Mie University
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- Ando Mei
- Graduate School of Bioresources, Mie University
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- Mizoguchi Yohei
- Graduate School of Bioresources, Mie University
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- Shimada Hiroto
- Graduate School of Bioresources, Mie University
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- Ohira Keima
- Graduate School of Bioresources, Mie University
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- Kagaya Yasuaki
- Advanced Science Research Promotion Center, Mie University
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- Higashiyama Tetsuya
- Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University Graduate School of Science, Nagoya University Graduate School of Science, The University of Tokyo
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- Takayama Seiji
- Graduate School of Agriculture and Life Sciences, The University of Tokyo
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- Watanabe Masao
- Graduate School of Life Sciences, Tohoku University
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- Suwabe Keita
- Graduate School of Bioresources, Mie University
Bibliographic Information
- Other Title
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- Two aquaporins, SIP1;1 and PIP1;2, mediate water transport for pollen hydration in the Arabidopsis pistil
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Abstract
<p>Pollination is the crucial initial step that brings together the male and female gametophytes, and occurs at the surface of the stigmatic papilla cell in Arabidopsis thaliana. After pollen recognition, pollen hydration is initiated as a second critical step to activate desiccated mature pollen grains for germination, and thus water transport from pistil to pollen is essential for this process. In this study, we report a novel aquaporin-mediated water transport process in the papilla cell as a control mechanism for pollen hydration. Coupled with a time-series imaging analysis of pollination and a reverse genetic analysis using T-DNA insertion Arabidopsis mutants, we found that two aquaporins, the ER-bound SIP1;1 and the plasma membrane-bound PIP1;2, are key players in water transport from papilla cell to pollen during pollination. In wild type plant, hydration speed reached its maximal value within 5 min after pollination, remained high until 10–15 min. In contrast, sip1;1 and pip1;2 mutants showed no rapid increase of hydration speed, but instead a moderate increase during ∼25 min after pollination. Pollen of sip1;1 and pip1;2 mutants had normal viability without any functional defects for pollination, indicating that decelerated pollen hydration is due to a functional defect on the female side in sip1;1 and pip1;2 mutants. In addition, sip1;1 pip1;2 double knockout mutant showed a similar impairment of pollen hydration to individual single mutants, suggesting that their coordinated regulation is critical for proper water transport, in terms of speed and amount, in the pistil to accomplish successful pollen hydration.</p>
Journal
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- Plant Biotechnology
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Plant Biotechnology 38 (1), 77-87, 2021-03-25
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390287462799979776
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- NII Article ID
- 130008002865
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- NII Book ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 031463288
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed