Establishment and Characterization of the Human Tongue Squamous Cell Carcinoma Cell Line NOKT-1

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  • Sakuma Kaname
    Department of Oral & Maxillofacial Surgery, Nippon Dental University School of Life Dentistry at Niigata
  • Takahashi Haruka
    Department of Oral & Maxillofacial Surgery, Nippon Dental University School of Life Dentistry at Niigata
  • Kii Tomoyuki
    Course of Clinical Science, Field of Oral and Maxillofacial Surgery and Systemic Medicine, Oral and Maxillofacial Surgery, Nippon Dental University Graduate School of Life Dentistry at Niigata
  • Watanabe Miho
    Department of Oral & Maxillofacial Surgery, Nippon Dental University School of Life Dentistry at Niigata
  • Tanaka Akira
    Department of Oral & Maxillofacial Surgery, Nippon Dental University School of Life Dentistry at Niigata Course of Clinical Science, Field of Oral and Maxillofacial Surgery and Systemic Medicine, Oral and Maxillofacial Surgery, Nippon Dental University Graduate School of Life Dentistry at Niigata

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<p>The squamous cell carcinoma cell line NOKT-1 was successfully established from the right tongue of a 74-yearold Japanese man. Pathological diagnosis of the original tumor was moderately differentiated squamous cell carcinoma. NOKT-1 cells were transplanted subcutaneously into nude mice and xenograft was formed. In addition, the NOKT-1-XG cell line was established from the transplanted tumor of NOKT-1 cells. NOKT-1 cells and NOKT-1-XG cells were epithelial neoplastic and pleomorphic cells, which were similar. Immunocytochemistry revealed that NOKT-1 and NOKT-1-XG cells were CK17 and human mitochondria positive. To authenticate the NOKT-1 cell line and NOKT-1-XG cell line, we examined cross-contamination with other cell lines using short tandem repeat analysis, the results of which showed that NOKT-1 and NOKT-1-XG are new cell lines. Four of the 16 loci, corresponding to 25%, were different between these two cell lines, which indicates that the NOKT-1 genome was altered by transplantation. Moreover, in AM, NOKT-1 did not have a Y chromosome, whereas NOKT-1-XG had. Despite the genetic differences, a collagen gel droplet-embedded culture drug susceptibility test demonstrated that NOKT-1 cells derived from the original tumor and the NOKT-1-XG cell line had the same sensitivity. This cell line could be very useful for the development of immunotherapy and chemotherapy regimens and research on cancer etiology.</p>

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