A Study of a Novel Infected Root Canal System Model Using Porcine Extracted Teeth
-
- Masanori FUJITA
- Department of Endodontics, School of Dentistry, Aichi Gakuin University
-
- Naoya HIGUCHI
- Department of Endodontics, School of Dentistry, Aichi Gakuin University
-
- Kyoko INAMOTO
- Department of Endodontics, School of Dentistry, Aichi Gakuin University
-
- Naoki HORIBA
- Department of Endodontics, School of Dentistry, Aichi Gakuin University
Bibliographic Information
- Other Title
-
- ブタ抜去歯を用いた新しい感染根管モデルの検討
Search this article
Abstract
<p> Purpose: In this study, we examined the usefulness of a new model of infected root canals for use as an in vitro experimental system, which was prepared by bacterial infection in the root canals of porcine extracted teeth.</p><p> Methods: Following the extraction of porcine mandibular molars, their roots were divided into mesial and distal groups. Cleaning and shaping of the root canal were performed with instrumentation up to #60, in accordance with conventional methods. Root canals were filled with a suspension of Enterococcus faecalis cells (containing an approximately consistent number of bacteria) and cultured aerobically at 37°C for 21 days. The following analyses were performed before, immediately after, and 2 days after root canal irrigation with sodium hypochlorite solution: ①comparison of the number of E. faecalis in the root canal, using the serial dilution method; and ②SEM observation of the root canal surface.</p><p> Results: Analysis using the serial dilution method showed that the number of E. faecalis decreased below the detection limit immediately after root canal irrigation with sodium hypochlorite solution; however, it had returned to a level comparable with the saline control at 2 days after irrigation. Furthermore, SEM observation showed no biofilm-like formation on the root canal wall surface immediately after irrigation with sodium hypochlorite solution, although it was again evident on the root canal wall surface at 2 days after irrigation.</p><p> Conclusion: It was suggested that this infected root canal system model using porcine extracted teeth has potential for use as an in vitro experimental system in the endodontic field.</p>
Journal
-
- The Japanese Journal of Conservative Dentistry
-
The Japanese Journal of Conservative Dentistry 64 (2), 133-140, 2021
The Japanese Society of Conservative Dentistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390287860632487680
-
- NII Article ID
- 130008032144
-
- ISSN
- 21880808
- 03872343
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed