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A Study of a Novel Infected Root Canal System Model Using Porcine Extracted Teeth

DOI Open Access
  • Masanori FUJITA
    Department of Endodontics, School of Dentistry, Aichi Gakuin University
  • Naoya HIGUCHI
    Department of Endodontics, School of Dentistry, Aichi Gakuin University
  • Kyoko INAMOTO
    Department of Endodontics, School of Dentistry, Aichi Gakuin University
  • Naoki HORIBA
    Department of Endodontics, School of Dentistry, Aichi Gakuin University

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Other Title
  • ブタ抜去歯を用いた新しい感染根管モデルの検討

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Abstract

<p> Purpose: In this study, we examined the usefulness of a new model of infected root canals for use as an in vitro experimental system, which was prepared by bacterial infection in the root canals of porcine extracted teeth.</p><p> Methods: Following the extraction of porcine mandibular molars, their roots were divided into mesial and distal groups. Cleaning and shaping of the root canal were performed with instrumentation up to #60, in accordance with conventional methods. Root canals were filled with a suspension of Enterococcus faecalis cells (containing an approximately consistent number of bacteria) and cultured aerobically at 37°C for 21 days. The following analyses were performed before, immediately after, and 2 days after root canal irrigation with sodium hypochlorite solution: ①comparison of the number of E. faecalis in the root canal, using the serial dilution method; and ②SEM observation of the root canal surface.</p><p> Results: Analysis using the serial dilution method showed that the number of E. faecalis decreased below the detection limit immediately after root canal irrigation with sodium hypochlorite solution; however, it had returned to a level comparable with the saline control at 2 days after irrigation. Furthermore, SEM observation showed no biofilm-like formation on the root canal wall surface immediately after irrigation with sodium hypochlorite solution, although it was again evident on the root canal wall surface at 2 days after irrigation.</p><p> Conclusion: It was suggested that this infected root canal system model using porcine extracted teeth has potential for use as an in vitro experimental system in the endodontic field.</p>

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