Recommendation of the Use of EDTA-containing Tubes for the Measurement of HbA1c Using a Layer of Erythrocytes Obtained after Centrifugation
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- Kuwa Katsuhiko
- Japan Reference Measurement Institute
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- Okahashi Mikiko
- Institute of Biopathological Medicine
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- Sato Asako
- Clinical Laboratory, Tokyo Women's Medical University
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- Namba Mitsuyoshi
- Takarazuka City Hospital
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- Maeda Shiro
- Department of Advanced Genomic and Laboratory Medicine, Graduate School of Medicine, University of the Ryukyus
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- Murakami Masami
- Department of Clinical Laboratory Medicine, Graduate School of Medicine, Gunma University
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- Meguro Shu
- Department of Internal Medicine, Division of Endocrinology, Metabolism and Nephrology, Keio University School of Medicine
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- Nishio Yoshihiko
- Department of Diabetes and Endocrine Medicine, Kagoshima University Graduate School of Medical and Dental Sciences
Bibliographic Information
- Other Title
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- 遠心処理後に測定するHbA1c測定法での採血管の取扱い(EDTA入り採血管の推奨)について
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Abstract
<p>Transportation of whole blood samples maintained in cold conditions for up to 48 hours out of the laboratory may lead to hemolysis. This consequently results in a lower measured HbA1c value when a layer of erythrocytes obtained by centrifugation of the hemolytic sample is used for an analysis, including analyses by some HPLC-, enzymatic- and immuno-assay systems. The use of NaF-containing tubes that are suitable for the measurement of glucose concentration, makes hemolysis easy. Thus, the committee on Standardization of Laboratory Testing Related to Diabetes Mellitus of the Japan Diabetes Society recommends the use of EDTA- containing tubes for the measurement of HbA1c using a layer of erythrocytes harvested by centrifugation.</p>
Journal
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- Journal of the Japan Diabetes Society
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Journal of the Japan Diabetes Society 64 (5), 336-339, 2021-05-30
THE JAPAN DIABETES SOCIETY
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Details 詳細情報について
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- CRID
- 1390288215572362496
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- NII Article ID
- 130008045891
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- ISSN
- 1881588X
- 0021437X
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed