The Role of Brain Methamidophos in Acephate Poisoning in Mice

DOI IR Web Site Web Site PubMed 18 References
  • TANAKA Toshiko
    Department of Forensic Medicine, School of Medicine, University of Occupational and Environmental Health, Japan
  • SATO Hiroaki
    Department of Forensic Medicine, School of Medicine, University of Occupational and Environmental Health, Japan
  • YOSHIDA Kosho
    Department of Forensic Medicine, School of Medicine, University of Occupational and Environmental Health, Japan
  • KASAI Kentaro
    Department of Forensic Medicine, School of Medicine, University of Occupational and Environmental Health, Japan

Bibliographic Information

Other Title
  • マウスのアセフェート中毒での脳メタミドホスの役割

Search this article

Description

<p>We gave mice a 540 mg/kg dose of LD50 acephate, followed by an assessment of acephate, methamidophos (MP), and choline esterase (ChE) activity for up to 4 hours (hr) in order to investigate the time course of acephate intoxication. At 1 hr, the blood acephate and MP levels were 428 ± 90 µg/ml (mean ± SEM) and 4.2 ± 0.4 µg/ ml, respectively. The liver acephate levels were similar to those in the blood, but the liver MP levels were approximately 3.5 times that of the blood at 1 hr. The brain MP level tended to be higher than the blood MP at 1 hr. These levels decreased gradually over 4 hr, but the brain acephate and MP levels surpassed the blood levels significantly at 4 hr, and after 2 hr, respectively. Serum, liver, cerebrum, cerebellum, and brainstem cholinesterase activity (ChE) were inhibited at 1 hr, and remained inhibited in all but the cerebellum until the end of the experiment. The obtained data were applied to previously reported autopsy cases of acephate intake. Experimental data suggest that brain MP is involved in acute acephate-induced poisoning, even after a reduction in blood acephate. In autopsy cases with suspected acephate poisoning, the MP level in the brain should be considered in addition to the ChE activity to diagnose the cause of death.</p>

Journal

  • Journal of UOEH

    Journal of UOEH 43 (2), 197-203, 2021-06-01

    The University of Occupational and Environmental Health, Japan

References(18)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top