タンパク質結晶化に応用可能な新規小型抗体フォーマットFv-claspの開発

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  • Development of a Novel Antibody Fragment Format ‘Fv-clasp’ Applicable to Protein Crystallization
  • タンパクシツ ケッショウカ ニ オウヨウ カノウ ナ シンキ コガタ コウタイ フォーマット Fv-clasp ノ カイハツ

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<p>In the protein crystallography, antibodies are frequently used as ‘crystallization chaperones’, where their binding facilitates production of high-quality diffracting crystals of complex macromolecules that are otherwise resistant to crystallization. To develop an ideal antibody fragment, we have designed a novel antibody fragment format, called ‘Fv-clasp’, that is a fusion of an anti-parallel coiled-coil structure derived from the hMst1 SARAH domain to the Fv fragment of an antibody. We have demonstrated that Fv-clasp has superior properties over conventional antibody fragments including Fab and single-chain Fv（scFv）in terms of producibility, stability, and crystallizability.</p>

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