A Simplified Method for Predicting the Effect of Temperature on the Separation Performance by Chromatography

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  • NOZAKI Shinya
    Graduate School of Sciences and Technology for Innovation, Yamaguchi University
  • YOSHIMOTO Noriko
    Graduate School of Sciences and Technology for Innovation, Yamaguchi University Biomedical Engineering Center (YUBEC), Yamaguchi University
  • YAMAMOTO Shuichi
    Graduate School of Sciences and Technology for Innovation, Yamaguchi University Biomedical Engineering Center (YUBEC), Yamaguchi University

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  • クロマトグラフィー分離の温度依存性の簡単な推算方法

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Abstract

<p>A simplified method for predicting the effect of temperature on the separation performance by chromatography was developed. As a model separation system, two polyphenol component separation by polymer-resin chromatography with ethanol-water mobile phase was chosen. HETP values as a function of mobile phase velocity for a wide range of temperatures 15-45°C were well correlated by a single universal curve in the dimensionless HETP and the dimensionless velocity plots. A simplified equation that can predict the resolution was derived. The equation includes the temperature dependence of the distribution coefficients and the molecular diffusion coefficients. The relative separation performance (resolution) calculated by this equation showed that the existence of the maximum resolution depends on the temperature dependence of the distribution coefficients. Even when the resolution decreases with temperature, higher temperature operations may be advantageous as both the separation time and the separation volume decrease.</p>

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