Development of a simultaneous detection method for GAD and IA-2 antibodies

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  • Kawano Tomoharu
    Division of Functional Food Chemistry, Institute for Health Science, Tokushima Bunri University
  • Numata Satoshi
    Dept. of Nutrition, University of Kochi
  • Kuroda Akio
    Diabetes Therapeutics and Research Center,The University of Tokushima.All Rights Reserved.
  • Yasuda Tetsuyuki
    Department of Diabetes and Endocrinology, Osaka Police Hospital
  • Miyashita Kazuyuki
    Department of Metabolic Medicine,Osaka University Graduate School of Medicine
  • Sakamoto Fumie
    Department of Metabolic Medicine,Osaka University Graduate School of Medicine
  • Katakami Naoto
    Department of Metabolic Medicine,Osaka University Graduate School of Medicine
  • Matsuoka Takaaki
    Department of Metabolic Medicine,Osaka University Graduate School of Medicine
  • Munehide Matsuhisa
    Diabetes Therapeutics and Research Center,The University of Tokushima.All Rights Reserved.
  • Hashida Seiichi
    Department of Diabetes and Molecular Genetics, Ehime University Graduate School of Medicine.

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Other Title
  • GAD抗体とIA-2抗体の2種抗体同時検出法の開発
  • GAD コウタイ ト IA-2 コウタイ ノ 2シュ コウタイ ドウジ ケンシュツホウ ノ カイハツ

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Abstract

<p>Development of a simultaneous detection method for GAD and IA-2 antibodiesTomoharu Kawano, Satoshi Numata, Akio Kuroda, Tetsuyuki Yasuda, Kazuyuki Miyashita,Fumie Sakamoto, Naoto Katakami, Takaaki Matsuoka, Munehide Matsuhisa and Seiichi HashidaSummaryObjective: Detection of pancreatic islet-associated autoantibodies （GAD antibody, IA-2 antibody） is useful for diagnosis of type 1 diabetes and prediction of onset. However, since each antibody has a high positive rate depending on age of onset and duration of disease, single antibody detection may misidentify positive individuals. Therefore, a simultaneous detection method for GAD and IA-2 antibodies was developed and reported.Methods: The simultaneous detection method was verified using model serum （24 specimens） prepared from positive serum and serum of diabetic patients （28 specimens）. For detection of GAD and IA-2 antibodies, an ultra-sensitive ELISA （ICT-EIA） method was used.Results and Conclusion: In model serum, the measured/expected ratio was 71-136％ with a predictive value of 24/24 （100％） by the simultaneous detection method. Further, this method showed higher values of sensitivity （57.1％） and specificity （100％） in sera of type 1 diabetic patients compared to single measurement of GAD antibody and IA-2 antibody. Furthermore, the predictive value for positive and negative obtained by two independent measurements was 28/28 （100％）. Specimens that were positive for either one or two antibodies could be judged as positive using this simultaneous detection method, indicating its usefulness.</p>

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