Construction of Hepatic Vascular Model and Toxicity Assessment System that Can Predict DILI Compounds

DOI
  • NAITO Yasuyuki
    Technical Research Institute, TOPPAN PRINTING CO., LTD. Joint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduate School of Engineering, Osaka University
  • YOSHINOUCHI Yuka
    Technical Research Institute, TOPPAN PRINTING CO., LTD. Joint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduate School of Engineering, Osaka University
  • KOHARA Hiroshi
    Drug Safety Research and Evaluation, Takeda Pharmaceutical Company Limited
  • KITANO Shiro
    Technical Research Institute, TOPPAN PRINTING CO., LTD. Joint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduate School of Engineering, Osaka University
  • IRIE Shinji
    Joint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduate School of Engineering, Osaka University
  • MATSUSAKI Michiya
    Department of Applied Chemistry, Graduate School of Engineering, Osaka University Joint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduate School of Engineering, Osaka University

Bibliographic Information

Other Title
  • 血管構造を有する類肝組織の構築、およびDILI化合物を予測可能な毒性評価系の検討

Abstract

<p>We constructed a hepatic vascular model for predicting hepatotoxicity by using our unique tissue-engineering method. Briefly, we created a viscous body by suspending cells with heparin and collagen. The viscosity can induce cell-cell adhesion suitable for constructing a hepatic tissue. We constructed the vascularized tissue whose hepatocytes ratio is 65% (close to in vivo). We confirmed the predictive performance of DILI by treating typical DILI compounds. Notably, we could predict the toxicity of Monocrotaline by vessel analysis with higher sensitivity than usual viability assay.</p>

Journal

Details 詳細情報について

  • CRID
    1390289011244372480
  • NII Article ID
    130008073843
  • DOI
    10.14869/toxpt.48.1.0_p-41
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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