Seedling production method of <i>Ulva prolifera</i> using ground fragments with addition of extract from the thalli

DOI
  • KITA Fumiya
    Education and Research Center for Aquascience, Faculty of Bioscience and Bioindustry, Tokushima University Kankyou Bousai Co., Ltd.
  • DAN Akinori
    Education and Research Center for Aquascience, Faculty of Bioscience and Bioindustry, Tokushima University
  • HAMANO Tatsuo
    Education and Research Center for Aquascience, Faculty of Bioscience and Bioindustry, Tokushima University
  • SAITO Minoru
    Education and Research Center for Aquascience, Faculty of Bioscience and Bioindustry, Tokushima University
  • OKA Naohiro
    Education and Research Center for Aquascience, Faculty of Bioscience and Bioindustry, Tokushima University

Bibliographic Information

Other Title
  • 藻体抽出液を細断細胞に添加するスジアオノリ種苗生産法

Description

Stabilization of seedling production is an urgent issue in the land-based cultivation of Ulva prolifera. In this study, we established a new seedling production method, in which branching formation from ground fragments is stimulated by addition of the Solution Extracted from Homogenized Thalli (SHET) of U. prolifera. The cells in the fragments cultured with SHET differentiated into six types of cells: swelled cells, branch cells, rhizoid cells, matured cells, empty cells, or dead cells. As the amount of the added SHET increased, the proportion of the fragments with branch cells increased. The branch cells grew normally, forming leaves after 7 days of culture period. Within the tested settings, the rate of branch formation increased accordingly to the extension of storage period and increase in culture temperature. We recommend storage period of 6 weeks and incubation temperature of 25℃ for seedling production, as the increase of maturing cells at 30℃ makes seedling production unstable, especially after extended storage period.

Journal

  • Algal Resources

    Algal Resources 12 (1), 11-20, 2019

    The Japanese Society of Applied Phycology

Keywords

Details 詳細情報について

  • CRID
    1390289068497660032
  • NII Article ID
    130008074392
  • DOI
    10.20804/jsap.12.1_11
  • ISSN
    24238473
    18833284
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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