Determination of Albendazole Metabolite in Livestock Products Using LC-MS/MS

Okabe Ryo, Kubota Akiko, Nemoto Satoru, Aoyagi Mitsutoshi

doi:10.3358/shokueishi.62.113

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LC-MS/MSを用いた畜産物中のアルベンダゾール代謝物の分析法
LC-MS/MS オモチイタチクサンブツチュウノアルベンダゾールタイシャブツノブンセキホウ

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Abstract

<p>A method for determining albendazole metabolite (metabolite I) in livestock products using LC-MS/MS was proposed. Livestock samples were hydrolyzed with 6 mol/L HCl at 110℃ for an hour and defatted with ethyl acetate and n-hexane (1 : 1, v/v) mixture. Metabolite I was extracted with acetonitrile from the sample, and the extracts were salted out under basic conditions, allowing the acetonitrile layer to separate. The acetonitrile solution was cleaned up using a cartridge column packed with divinylbenzene-N-vinylpyrolidone copolymer bearing sulfo groups. The HPLC separation was conducted on an Inertsil ODS-4 column with a gradient formed from water containing 0.05% (v/v) formic acid and acetonitrile containing 0.05% (v/v) formic acid. To detect metabolite I, tandem mass spectrometry with positive ion electrospray ionization was used. Truenesses (n=5) of metabolite I from cattle meat, fat, liver, and milk spiked at the maximum residue limits or the 0.01 mg/kg were in the range from 83.6 to 97.9%, and the relative standard deviations were from 1.6 to 6.1%.</p>

Journal

Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)

Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 62 (4), 113-118, 2021-08-25

Japanese Society for Food Hygiene and Safety

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