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Influence of the storage temperature on the nucleic acid quality in liquid-based cytology samples prepared using fixed in CytoRich Red
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- ABE Hideyuki
- Department of Diagnostic Pathology, Kurume University Hospital
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- KAWAHARA Akihiko
- Department of Diagnostic Pathology, Kurume University Hospital
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- SADASHIMA Eiji
- Life Science Research Institute, Saga-ken Medical Center Koseikan
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- MURATA Kazuya
- Department of Diagnostic Pathology, Kurume University Hospital
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- TAKASE Yorihiko
- Department of Diagnostic Pathology, Kurume University Hospital
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- MAKINO Ryo
- Department of Diagnostic Pathology, Kurume University Hospital
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- YOSHIDA Tomoko
- Department of Diagnostic Pathology, Kurume University Hospital
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- FUKUMITSU Chihiro
- Department of Diagnostic Pathology, Kurume University Hospital
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- SHINODA Yukako
- Department of Diagnostic Pathology, Kurume University Hospital
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- NAITO Yoshiki
- Department of Diagnostic Pathology, Kurume University Hospital
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- AKIBA Jun
- Department of Diagnostic Pathology, Kurume University Hospital
Bibliographic Information
- Other Title
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- サイトリッチレッドを用いた LBC 検体の保存温度における核酸品質への影響
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Description
<p>Objective : Nucleic acid analysis is an important diagnostic tool for the diagnosis of disease or determining treatment options. The aim of this study was to examine the effects of the cell sample storage temperature on the nucleic acid quality in liquid-based cytology samples prepared using fixed in CytoRich Red.</p><p>Study Design : We investigated the effects of the storage temperature on the DNA yield and quality, as measured by the DNA integrity number (DIN) and purity (A260/A280). Culture cells (PC9) and clinical samples (n=11) were fixed overnight in CytoRich Red and then stored for up to 10 days at room temperature or at 4℃ in a refrigerator.</p><p>Results : The DNA quality of the PC9 cells fixed in CytoRich Red was adversely affected by storage at room temperature, but not by storage at 4℃ for up to 10 days. In the clinical samples also, the amount of extracted DNA decreased with storage at room temperature (p<0.001), whereas low temperature storage protected against DNA degradation in the samples, as assessed by measurement of the DIN and purity (A260/A280).</p><p>Conclusion : Since the storage temperature influences the nucleic acid quality of the samples, cytological samples should be stored under refrigeration at around 4℃.</p>
Journal
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- The Journal of the Japanese Society of Clinical Cytology
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The Journal of the Japanese Society of Clinical Cytology 60 (4), 212-218, 2021
The Japanese Society of Clinical Cytology
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Keywords
Details 詳細情報について
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- CRID
- 1390289332386377216
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- NII Article ID
- 130008087309
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- ISSN
- 18827233
- 03871193
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed