書誌事項
- タイトル別名
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- Detection of Endotoxin in Blood Components Using Limulus Test
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説明
In order to establish a detection system for endotoxin in blood products, we investigated for a method for sample pretreatment optimal for limulus test. In measuring endotoxin in blood products, an aliquot of plasma was 10 times diluted with surface-active agent and heat-treated at 80℃ for the purpose of removing inhibitory effect on endotoxin activity derived from serum component. Treated sample was then allowed to endotoxin analysis using a toxinometer MT-358(Wako)that yielded 100% recovery of endotoxin added to blood products. However, endotoxin activity in red cell-MAP supernatant was completely lost after heat-treatment, rendering the pretreatment method inadequate for measurement of endotoxin in red cell-MAP. Impaired endotoxin activity, however, possibly caused by a heat treatment under a very low protein concentration, was found to be restored after diluting the sample 30-40 times with endotoxin-free water without heating, yielding a recovery of more than 90%.
収録刊行物
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- 医科器械学
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医科器械学 68 (9), 375-380, 1998-09-01
一般社団法人 日本医療機器学会
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詳細情報 詳細情報について
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- CRID
- 1390289332387796864
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- NII論文ID
- 110002515667
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- NII書誌ID
- AN00013123
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- ISSN
- 18814875
- 0385440X
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可