Evaluating the effects of differentiation inducers on K562 cells by measuring electrorotation rate

DOI
  • Suzuki Masato
    Graduate School of Material Science, University of Hyogo, Hyogo, Japan
  • Takeuchi Rino
    Graduate School of Material Science, University of Hyogo, Hyogo, Japan
  • Yasukawa Tomoyuki
    Graduate School of Material Science, University of Hyogo, Hyogo, Japan

Bibliographic Information

Other Title
  • 細胞の電気回転速度を指標とした分化誘導剤の評価

Abstract

<p>The electrorotation (ROT) rates of K562 cells accompanied by erythroid differentiation were measured to identify the differentiation status induced by three kinds of agents, which were butyric acid (BA), nicotinic acid (NA), and isonicotinic acid (INA). For induction of ROT, a novel electrorotation device with a microwell arranged on a center of polynomial electrode was fabricated. The polynomial electrode consisted of four electrodes arranged symmetrically around a point. The single K562 cell was trapped in the microwell and was subjected to ROT. The ROT rate increased with the differentiation of K562 cells, because the cytoplasm conductivity would increase with an increase of the concentration of iron ions to produce hemoglobin. The ROT rate could be utilized to estimate the stage of cell differentiation without labeling.</p>

Journal

Details 詳細情報について

  • CRID
    1390289765040546688
  • NII Article ID
    130008105237
  • DOI
    10.11239/jsmbe.annual59.320
  • ISSN
    18814379
    1347443X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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