The Teaching Material Development about the Effects of UV Radiation and Photoreactivation Using <i>Paramecium</i>

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  • ゾウリムシを用いた紫外線障害とその光回復効果を学ぶための教材開発
  • ゾウリムシを用いた紫外線傷害とその光回復効果を学ぶための教材開発
  • ゾウリムシ オ モチイタ シガイセン ショウガイ ト ソノ ヒカリ カイフク コウカ オ マナブ タメ ノ キョウザイ カイハツ

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<p>Ultraviolet (UV)-irradiated damage and photoreactivation in Paramecium tetraurelia (stock 51) were examined to introduce these phenomena into laboratory exercises in high school biology.</p><p>UV irradiation was carried out by a UV lamp (UV-B). After UV-irradiation at 500 J/m2 and 1,000 J/m2 the Paramecia were grown in the dark at 25°C for 6 days. The number of cells grown was higher with no radiation.</p><p>The Paramecium irradiated at 500 J/m2 and 100 J/m2 were exposed to fluorescent light (20,000 lx) for 24 hrs at 25°C, and photoreactivation effects were examined. This post-treatment with fluorescent light reduced the effects of UV radiation, remarkably. This result indicates that the UV-induced lethality was rescued by photoreactivation. However, the number of cells grown was higher with both UV and florescent light radiation.</p><p>We propose this experiment as a biology exercise in high schools, since it can be carried out with simple devices and results can be achieved in 6 days.</p>

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