Blastodisc morphology during first cleavage of the Oryzias latipes (medaka) fish egg

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The objectives of the study were a) to monitor changes in the morphology of the blastodisc of the fertilized medaka egg during the first cleavage, and b) to correlate these changes temporally to mitotic events. The morphology of the blastodisc was monitored by time-lapse video microscopy and an image analysis system. To see the boundary between the blastodisc and the yolk vacuole more clearly, we microinjected FITC-dextran into the yolk vacuole. To monitor mitotic events, we either microinjected Hoechst 33342 into living eggs or stained fixed eggs with Hoechst 33258; these eggs were monitored via a SIT camera and video monitor. We found that the first sign of cytokinesis is the formation of an internal furrow that forms at the surface between the blastodisc and the yolk vacuole. At 25℃, this furrow begins to form 3 minutes after the metaphaseanaphase transition and 7 minutes before the external furrow begins to form.

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