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One-step Removal of Proteases in a Commercial Lactase Preparation from <i>Kluyveromyces lactis</i> Using an Anion-exchange Membrane
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- Yoshikawa Jun
- Enzymes and Pharmaceuticals Laboratory, GODO SHUSEI Co., Ltd.
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- Shiota Kazuma
- Enzymes and Pharmaceuticals Laboratory, GODO SHUSEI Co., Ltd.
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- Horiguchi Hirofumi
- Enzymes and Pharmaceuticals Laboratory, GODO SHUSEI Co., Ltd.
Bibliographic Information
- Other Title
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- One-step removal of proteases in a commercial lactase preparation from Kluyveromyces lactis using an anion-exchange membrane
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Description
<p>To reduce proteases in a commercial lactase preparation from K. lactis, enzyme preparations were diluted by a buffer with KCl and the dilutions were flushed through a weak anion-exchange membrane. For KCl concentration in the range of 0.20 to 0.25 M, more than 90% of lactase was recovered and protease level was decreased to less than 20%. Purified and pre-purified lactase solutions added in excess to milk and incubated for 3 months at 30 °C showed degraded milk protein bands on SDS-PAGE by treatment with the pre-purified lactase but not purified lactase. A one-step purification method for protease removal from the K. lactis lactase preparation was performed only by flushing the solution through an anion-exchange membrane.</p>
Journal
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- Food Science and Technology Research
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Food Science and Technology Research 26 (1), 65-68, 2020
Japanese Society for Food Science and Technology
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Details 詳細情報について
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- CRID
- 1390290699451770368
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- ISSN
- 18813984
- 13446606
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
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- Abstract License Flag
- Disallowed