Seed Abortion in Crosses between Diploid and Tetraploid Grapes (Vitis vinifera and V. complex) and Recovery of Triploid Plants through Embryo Culture

DOI 機関リポジトリ (HANDLE) 被引用文献1件 オープンアクセス
  • 若菜 章
    Laboratory of Horticultural Science,Division of Agricultural Botany,Department of Plant Resources,Faculty of Agriculture,Kyushu University
  • 比良松 道一
    Laboratory of Horticultural Science,Division of Agricultural Botany,Department of Plant Resources,Faculty of Agriculture,Kyushu University
  • Park Sung Min
    Laboratory of Horticultural Science,Division of Applied Plant Sciences,College of Agriculture and Life Scciences,Kangwon Nation University
  • 花田 信章
    Laboratory of Fruit Tree Science, University Farm, Faculty of Agriculture, Kyushu University
  • 福留 功
    Laboratory of Fruit Tree Science, University Farm, Faculty of Agriculture, Kyushu University
  • 安河内 幸一
    Laboratory of Fruit Tree Science, University Farm, Faculty of Agriculture, Kyushu University

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説明

Interploid crosses between two diploid and two tetrapkiod grape cultivars were carried out to produce abortive seeds with underdeveloped triploid embryos. Growth regulations influencing the recoveryof triploid plants from the underdeveloped embryos were individually examined in vitro using MS medium. Of six growth regulations added to the medium, malt extract (25-1600mg/l), casein hydrolysate (20-1500mg/l), BA (0.01-1.25mg.l) and GA[3] (0.1-1mg/l) did not show obvious effect on the recovery, whereas IAA and NAA showed small promotiveeffect at the concentration of 0.25 and 1.25mg.l and 0.01mg/l respectively. Secondly, 23 interploid crosses with four diploid and hour tetraploid grape cultivars were carried out reciprocally to produce triploid plants through embryo culture using MS medium supplemented with 1mg/l GA[3] and 100mg/l casein hydrolysate. In the 2x*4x crosses, 50 triploid seedlings were successfully recovered from 7.3% of inderdeveloped embryos culutured, while 33 seedlings were recovered from 2.8% of those in the reciprocals. Of the total of 88 seedling recovered, 38 were obtained through secondary embryo formation in vitro. From these results, it was suggested that the production rates of triploid plants from the interploid crosses increase with the aid of embryo culture, and that in this case in vitro formation of secondary embryos is an important key factor to increase the rates.

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詳細情報 詳細情報について

  • CRID
    1390290699660558976
  • NII論文ID
    110001800164
  • NII書誌ID
    AA00247166
  • DOI
    10.5109/4524
  • HANDLE
    2324/4524
  • ISSN
    00236152
  • 本文言語コード
    en
  • 資料種別
    departmental bulletin paper
  • データソース種別
    • JaLC
    • IRDB
    • Crossref
    • CiNii Articles
    • OpenAIRE

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