The Modification of Carboxyl Group of Lysozyme

DOI IR (HANDLE) Open Access
  • Hayashi Katsuya
    Laboratory of Biochemistry, Department of Agriculture, Kyushu University
  • Kugimiya Masayuki
    Laboratory of Biochemistry, Department of Agriculture, Kyushu University
  • Shimoda Tadahisa
    Laboratory of Biochemistry, Department of Agriculture, Kyushu University
  • Matsumoto Nobuya
    Laboratory of Biochemistry, Department of Agriculture, Kyushu University
  • Funatsu Masaru
    Laboratory of Biochemistry, Department of Agriculture, Kyushu University

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Description

Lysozyme molecule contains eleven carboxyl groups which can be classified by the titration behavior into four types, not titratable, with low pK-value, normal and with extraordinary high pK-value. Furthermore, X-ray crystallographic studies revealed that each carboxyl group can be characterized by the accessibility to the surrounding medium according to its position in the molecule in the crystalline state. The present experiments were carried out to clarify a correlation between a chemical reactivity and the physical state of the carboxyl groups by modifying them with condensing reagent carbodiimide and glycine derivatives. The results indicated that at low extent of the modification carboxyl groups were selectively modified, i.e., several carboxyl groups can be distinguished by their chemical reactivities.

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Details 詳細情報について

  • CRID
    1390290699661122432
  • NII Article ID
    110000017267
  • NII Book ID
    AA00247166
  • DOI
    10.5109/22813
  • HANDLE
    2324/22813
  • ISSN
    00236152
  • Text Lang
    en
  • Article Type
    departmental bulletin paper
  • Data Source
    • JaLC
    • IRDB
    • Crossref
    • CiNii Articles
    • OpenAIRE

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