Distribution of Isalia sinclairii in the forest soil on various season and soil depth.

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  • Ohga Shoji
    Division of Forest Environmental Sciences, Department of Agroenvironmental Sciences, Faculty of Agriculture, Kyushu University
  • Takano Katsuhiro
    Department of Forest and Forest Products Sciences, Graduate school of Bioresource and Bioenvironmental Sciences, Kyushu University
  • Sun Zhu
    Department of Forest and Forest Products Sciences, Graduate school of Bioresource and Bioenvironmental Sciences, Kyushu University

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  • ツクツクボウシタケ菌の森林土壌内での時空間分布
  • ツクツクボウシタケキン ノ シンリン ドジョウ ナイ デ ノ ジクウカン ブンプ

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Abstract

We tested the reliability and suitability of specific primer set (PC-F-GC/PC-R) by using the Denaturing gradient gel electrophoresis (DGGE) analysis for detecting I. sinclairii in the forest soil. Soil samples were taken from the sites with fruiting record of I. sinclairii. Total DNA was extracted from soil samples and analyzed with respect to the collecting phase and soil depth. The fungus of I. sinclairii was detected in June just before fruiting, and then peaked in August. Detection of the mycelia was declined to October and December. The mycelium of I. sinclairii was detected within a depth from 5 to 30 cm. The method developed in this study offers an effective tool for the elucidation of the biological characteristic of caterpillar fungi that has not fully been explained.

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