Identification of Genes that Predict Lymph Node Metastasis in Colorectal Cancer Cases

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  • Ueo Hiroki
    Department of surgery and science, Kyushu university beppu hospital | Department of surgery and science, Graduate school of medical science, Kyushu University
  • Takano Yuki
    Department of surgery and science, Kyushu university beppu hospital
  • Matsumura Tae
    Department of surgery and science, Kyushu university beppu hospital
  • Kurashige Junji
    Department of surgery and science, Kyushu university beppu hospital
  • Shinden Yoshiaki
    Department of surgery and science, Kyushu university beppu hospital
  • Eguchi Hidetoshi
    Department of surgery and science, Kyushu university beppu hospital
  • Sudo Tomoya
    Department of surgery and science, Kyushu university beppu hospital
  • Sugimachi Keishi
    Department of surgery and science, Kyushu university beppu hospital
  • Saeki Hiroshi
    Department of surgery and science, Graduate school of medical science, Kyushu University
  • Oki Eiji
    Department of surgery and science, Graduate school of medical science, Kyushu University
  • Morita Masaru
    Department of surgery and science, Graduate school of medical science, Kyushu University
  • Ikeda Tetsuo
    Department of surgery and science, Graduate school of medical science, Kyushu University
  • Maehara Yoshihiko
    Department of surgery and science, Graduate school of medical science, Kyushu University
  • Mimori Koshi
    Department of surgery and science, Kyushu university beppu hospital

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Other Title
  • 大腸癌のリンパ節転移を予測する遺伝子の同定
  • ダイチョウ ガン ノ リンパセツ テンイ オ ヨソク スル イデンシ ノ ドウテイ

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Abstract

Introduction : Currently, Endoscopic mucosal resection (EMR) and laparoscopic surgery with colorectal cancer (CRC) and has been expanding rapidly. In handling of colon cancer, adaptation of EMR is determined by the depth of tumor invasion. It is important to identify genes to predict lymph node metastasis in early CRC tumors precisely in a reproducible fashion to determine the adaptation of EMR treatment. We performed the comprehensive analysis of gene expression and genomic copy number simultaneously in CRC primary tumors to identify the bona-fide indicator of lymph node metastasis. Materials and Methods : We collected cancer cells specifically by Laser Microdissection (LMD) on 157 cases of primary colorectal cancer, and performed oligo microarrays for gene expression (GE) and aCGH for copy number aberration. As for candidate genes to be associated with lymph node metastasis, we examined reprodicibility by quantitative RT-PCR using cDNA created from the RNA extracted from 172 cases of CRC. Results : As for the association of lymph node metastasis, we found that 240 genes and 54 genes by aCGH and by oligo GE microarray, respectively. According to database of those two arrays, 501 genes were significantly correlated (correlation coefficient > 0.7) with each other, and we found that 11 out of 501 genes were identified as lymph node metastasis related genes with copy number alteration. Of these 11 genes, we focused on PCM1, MTUS1, ASAH1 on 8p22. Then, we confirmed that the decreased expression and genomic deletion of MTUS1 were observed in lymph node positive cases (p = 0.0195) in another subset of 172 cases of CRC. Conclusions : To measure the expression of MTUS1 of the tumor by PCR, we can predict the presence of lymph node metastasis. We expected that the loss of MTUS1 should be an important marker in determining the adaptation of endoscopic resection.

Journal

  • 福岡醫學雜誌

    福岡醫學雜誌 104 (12), 559-563, 2013-12-25

    Fukuoka Medical Association

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