-
- 園田 康平
- 九州大学大学院医学研究院眼科学分野
書誌事項
- タイトル別名
-
- Natural Immunity and Ocular Inflammation
- シゼン エンショウ ガ カンヨ スル ガン シッカン
この論文をさがす
抄録
We have focused on the role of innate immunity during the formation of “choroidal neovascularization (CNV) -related diseases”. Inflammation affects the formation and the progression of various vitreoretinal diseases. We performed a comprehensive analysis of inflammatory immune mediators in the vitreous fluids with diabetic macular edema, proliferative diabetic retinopathy, branch retinal vein occlusion, central retinal vein occlusion and rhegmatogenous retinal detachment. The concentrations of 20 soluble factors (nine cytokines, six chemokines, and five growth factors) were measured simultaneously by multiplex bead analysis system. Out of 20 soluble factors, three factors : interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant protein-1 (MCP-1) were significantly elevated in all groups of vitreoretinal diseases compared with control group. According to the correlation analysis in the individual patient’s level, these three factors that were simultaneously increased, did not show any independent upregulation in all the examined diseases. We also elucidated the role of natural killer (NK)T cells, which restricted CD1 molecule and participate in the innate immune response, in laser-induced experimental CNV. We examined CNV formation in independent two NKT cell-deficient mice, either CD1 knockout (KO) mice or Ja18 KO mice, and found that both KO mice showed significant reduction of experimental CNV. During the clinical process of CNV-related diseases, not only CNV formation, subretinal scaring is thought to be another important step. We thus established the experimental model of subretinal scaring by injecting peritoneal exudating macrophages into subretinal space. Subretinal fibrous tissue was observed by fundus scope in PEC-inoculated mice after seven days. The tissue was consisted of monotonous and low cell-density area, which expressed a-SMA with collagen synthesis. Because PEC-inoculated MCP-1 KO mice showed less amount of glial residual, not only exogenous macrophages, but also intrinsic macrophages are critical. Activated macrophages directly induced myofibrotic changes in RPE cells in vitro.
収録刊行物
-
- 福岡醫學雜誌
-
福岡醫學雜誌 107 (12), 205-212, 2016-12-25
福岡医学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390290699741479808
-
- NII論文ID
- 120006220523
-
- NII書誌ID
- AN00215478
-
- DOI
- 10.15017/1806768
-
- HANDLE
- 2324/1806768
-
- NDL書誌ID
- 028213284
-
- ISSN
- 0016254X
-
- 本文言語コード
- ja
-
- データソース種別
-
- JaLC
- IRDB
- NDL
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用可