lnteraction of ribonuclease F1 with various guanine nucleotides as studied by differential absorption spectra
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- Interaction of ribonuclease F1 with various guanine nucleotides as studied by difference absorption spectra
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lnteraction of various guanine and related nucleotides with ribonuclease Fl was studied by means of difference absorption spectra. Spectrophotometric titrations were carried out on a total of 16 nucleotides which were classined into three groups; (A)guanine and hypoxanthine nucleotides(6),(B)8-substituted guanine nucleotides (6) and(C) dinucleotides (4)(the numbers of nucleotides are shown in parentheses). Computer-aided data processing enabled determination of the difference molar absorbance coefficient △Emax and the dissociation constant of the ribonuclease F1-nucleotide complex Kd for each nucleotide. Among group A nucleotides,guanosine 2'-phosphate showed the lowest Kd (the highest affinity) and inosine 2'-phosphate the highest Kd (the lowest affinity).The difference between their binding free energy changes was estimated to reach 2.3 kcal/mol. Among group B nucleotides,8-aminoguanosine 2'-phosphate exhibited the lowest Kd,which was even lower than that ofguanosine 2'-phosphate. The dinerence in their binding free energy changes was l.0 kcal/mol. Group C nucleotides had higher Kd than their common component,guanosine 3'-phosphate,suggesting destabilizing effect on complex formation by an adjacent nucleotide. A11 these results were similar but not completely the same for those reported on ribonuclease TI.
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- 島根大学医学部紀要
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島根大学医学部紀要 32 1-6, 2009-12-01
島根大学医学部
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詳細情報 詳細情報について
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- CRID
- 1390290699794819200
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- NII論文ID
- 110008755138
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- NII書誌ID
- AA12049432
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- ISSN
- 1880084X
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- DOI
- 10.24568/6999
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- NDL書誌ID
- 10617256
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
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