Effect of Temperature on the Destruction of PC-3 Cells by Deformation
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- Takamatsu Hiroshi
- Institute of Advanced Material Study Kyushu University
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- Kumagae Norihiko
- Mitsui Chemicals, Inc.
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- Honda Hiroshi
- Institute of Advanced Material Study Kyushu University
Bibliographic Information
- Other Title
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- PC-3細胞の圧迫変形損傷に及ぼす温度の影響
- PC 3 サイボウ ノ アッパク ヘンケイ ソンショウ ニ オヨボス オンド ノ エイキョウ
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Abstract
Recent studies show that during slow freezing of cells, in addition to damage by so-called solution effect, the cells may be also injured by mechanical damage induced by ice crystal compression. A new experimental procedure was employed to develop a quantitative understanding of cell destruction by deformation with two parallel surfaces. The viability of deformed cells (prostatic carcinoma cell line PC-3) was measured at 0℃, 23℃ and 37℃, and six different nominal gaps between 32.3μm and 3.5μm. The relation between measured viability and gap size for 0℃ and 23℃ are identical. This may suggest that deformation damage is not related to the mechanical properties of the lipid membrane but the deformation of the cytoskelton.
Journal
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- 九州大学機能物質科学研究所報告
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九州大学機能物質科学研究所報告 14 (2), 105-109, 2000-12-25
Institute of Advanced Material Study Kyushu University
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Details 詳細情報について
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- CRID
- 1390290699812249984
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- NII Article ID
- 110006177569
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- NII Book ID
- AN10060378
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- ISSN
- 09143793
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- DOI
- 10.15017/7921
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- HANDLE
- 2324/7921
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- NDL BIB ID
- 5620032
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- CiNii Articles
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- Abstract License Flag
- Allowed