Novel Types of Diploidization in Ethanol-Tolerant Yeasts Delayed Homothallism and Endomitotic Diploidization Induced by Heat Treatment

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  • Hayashida Shinsaku
    Laboratory of Applied Microbiology, Faculty of Agriculture, Kyushu University
  • Tani Yoshinobu
    Laboratory of Applied Microbiology, Faculty of Agriculture, Kyushu University

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Other Title
  • エタノール耐性酵母の新規な二倍体化現象に関する研究 : 遅延性ホモタリズムおよび熱処理による同質二倍体化の発見と解析
  • エタノール耐性酵母の新規な2倍体化現象に関する研究--遅延性ホモタリズムおよび熱処理による同質2倍体化の発見と解析
  • エタノール タイセイ コウボ ノ シンキ ナ 2バイタイカ ゲンショウ ニ カ

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S.cerevisiae 1200 and S.cerevisiae wy2 were isolated as ethanol-tolerant yeast. Aerobically grown cells from spores enhanced ethanol tolerance. S. cerevisiae wy2 showed a novel type of life cycle, referred to as delayed homothallism. Diploidization was extraordinally imduced by the heat treatment(55℃, 10min) during spore germination. The obtained diploid cells with ability to mate were homozygous for mating types and auxotrophic markers. Thus the diploidization was concluded to be endomitotic events in germinating heated spore. A variety of polyploid cells including octaploid cells were constructed using a series of polyploid mater cells.

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