A computer program for estimating size distribution of spherical organelles from electron micrographs

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  • 球形オルガネラのサイズ分布を電子顕微鏡写真から推定するためのコンピュータープログラム
  • キュウケイ オルガネラ ノ サイズ ブンプ オ デンシ ケンビキョウ シャシン

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Abstract

Size distributions of intracellular organelles in an ultra-thin section are usually distorted when the section thickness is not negligible. A computer program is developed here to estimate true size distributions of spherical organelles from transmission electron micrographs of ultra-thin sections. The method is applied to determine size distributions of chromaffin granules isolated from bovine adrenal medullary cells as a basis for further physiological measurements.

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