Vitamin D Activates Various Gene Expressions, Including Lipid Metabolism, in C2C12 Cells
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- MIZUTANI Sako
- Laboratory of Molecular Nutrition, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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- OYABU Mamoru
- Laboratory of Molecular Nutrition, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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- YAMAMOTO Arisa
- Laboratory of Molecular Nutrition, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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- UCHITOMI Ran
- Laboratory of Molecular Nutrition, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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- SUGIMOTO Takumi
- Laboratory of Molecular Nutrition, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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- KAMEI Yasutomi
- Laboratory of Molecular Nutrition, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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説明
<p>Vitamin D is a fat-soluble molecule, well known for its role in regulating calcium homeostasis in bone. It has become increasingly clear that it also has important effects in many other organs, including the skeletal muscle. In order to gain insight into the role of vitamin D in the skeletal muscle, we performed microarray analysis using C2C12 myoblasts treated with 1,25-dihydroxyvitamin D (1,25(OH)2D), active form of vitamin D. We found multiple genes upregulated by 1,25(OH)2D. Some of them, i.e., vitamin D receptor (Vdr), diacylglycerol O-acyltransferase (Dgat1 and Dgat2, the rate limiting steps of triacylglycerol acylation), and vascular endothelial growth factor A (Vegfa), were previously reported to be upregulated by 1,25(OH)2D in C2C12 cells. RT-qPCR analysis confirmed increased mRNA levels of Rarres2, Dio2, Tgm2, Lpl, Mdfi, Igfbp3, Dgat1, Crabp2, Gadd45a, Vagfa, Dgat2, C3, Ldhb, Cebpa, Igfbp5, Mrc2, Vdr. Thus, many genes, including lipid metabolism genes as well as genes related to muscle functions, appear to be upregulated by 1,25(OH)2D in muscle cells.</p>
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- Ｊｏｕｒｎａｌ ｏｆ Ｎｕｔｒｉｔｉｏｎａｌ Ｓｃｉｅｎｃｅ ａｎｄ Ｖｉｔａｍｉｎｏｌｏｇｙ
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Ｊｏｕｒｎａｌ ｏｆ Ｎｕｔｒｉｔｉｏｎａｌ Ｓｃｉｅｎｃｅ ａｎｄ Ｖｉｔａｍｉｎｏｌｏｇｙ 68 (1), 65-72, 2022-02-28
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