Mild Oxidative Stress on the PC12 Cell Line: A Simple Model of Chronic Neural Tissue Oxidative Damage

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  • SATO Hiroaki
    Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture
  • MAGOON Joanne
    Biotechnology Research Institute, National Research Council Canada
  • TAKANO Katsumi
    Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture
  • KONISHI Yasuo
    Biotechnology Research Institute, National Research Council Canada

Bibliographic Information

Other Title
  • PC12細胞に対する緩慢な酸化ストレス：神経組織における慢性的酸化損傷の簡易モデル

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<p>　Here we constructed a mild oxidative system using the PC 12 cell line for ex vivo screening of antioxidants that are effective in reducing chronic oxidative damage in neural tissues. Nerve growth factor-induced differentiated PC 12 cells were exposed to various concentrations of hydrogen peroxide (H2O2) for six days. For three days after treatment initiation, the survival rate of cells exposed to less than 50 μM H2O2 was similar to that of untreated cells, whereas H2O2 concentrations higher than 50 μM exhibited a significant lethal effect. However, six days after treatment initiation, the survival rate of cells exposed to 50 μM H2O2 decreased significantly. This condition may thus mimic natural chronic oxidative damage of neural tissues. A well-known antioxidant, N-acetyl-L-cysteine, was added either before, during, or after H2O2 exposure to rescue cells from oxidative damage. N-acetyl-L-cysteine addition before and during oxidant treatment significantly rescued cells from damage, while addition after H2O2 treatment exhibited only a weak rescue effect. This simple method may be applied to screen for natural and artificial antioxidants with further modifications.</p>

Journal

  • Food Preservation Science

    Food Preservation Science 37 (3), 115-119, 2011

    Japan Association of Food Preservation Scientists

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