Highly conserved region 141-168 of the NS1 protein is a new common epitope region of dengue virus

DOI Web Site Web Site 被引用文献2件
  • Masrinoul Promsin
    Department of Virology, Research Institute for Microbial Diseases, Osaka University, Japan
  • Diata Magot Omokoko
    Department of Virology, Research Institute for Microbial Diseases, Osaka University, Japan
  • Pambudi Sabar
    Department of Virology, Research Institute for Microbial Diseases, Osaka University, Japan
  • Limkittikul Kriengsak
    Department of Tropical Pediatrics, Faculty of Tropical Medicine, Mahidol University, Thailand
  • Ikuta Kazuyoshi
    Department of Virology, Research Institute for Microbial Diseases, Osaka University, Japan Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, Thailand
  • Kurosu Takeshi
    Department of Virology, Research Institute for Microbial Diseases, Osaka University, Japan Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, Thailand

書誌事項

タイトル別名
  • Highly Conserved Region 141‒168 of the NS1 Protein Is a New Common Epitope Region of Dengue Virus

この論文をさがす

抄録

<p>Dengue virus (DENV) nonstructural protein 1 (NS1) is a major target of humoral immunity in patients and is believed to be involved in DENV pathogenesis. In addition, NS1 is a diagnostic target as it is secreted, and circulates, in patients’ plasma at an early stage of viral infection. In this study, we aimed to identify common epitope regions for all serotypes by preparation of mouse monoclonal antibodies (MAbs) against NS1. A total of 10 out of the 20 hybridoma clones which were specific to DENV produced MAbs that recognized NS1. These MAbs mapped to three regions of DENV-2 NS1, namely amino acids 1‒40 (epitope region 1), 141‒168 (epitope region 2), and 267‒312 (epitope region 3). Epitope region 2 was recognized by both complex-specific (2H11 and 3C4) and subcomplex-specific MAbs (4E5 and 5G12), whereas epitope regions 1 and 3 were recognized by subcomplex-specific MAbs (5E2, 1A5, and 3F10) only. These epitope regions were found to be highly conserved among all four serotypes of DENV by sequence analysis and database comparison. The MAbs against these epitope regions, especially 2H11 and 3C4, could therefore be valuable diagnostic tools.<tt> </tt></p>

収録刊行物

被引用文献 (2)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ