Comparison of cytokine removal by PMMA and AN69ST membrane filters in the pig sepsis model

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  • Yamazaki Akio
    Department of Clinical Engineering, Aomori Jikeikai Hospital
  • Tubo Toshihito
    Chinese Medicine, Aizu Medical Center Fukushima Medical University
  • Miyasho Taku
    Animal Nutrition Unit （County Veterinary Nursing Class in Veterinary Insurance）, Rakuno Gakuen University
  • Hirota Kazuyoshi
    Intensive Care Unit, Hirosaki University Hospital

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Other Title
  • ブタ敗血症modelを用いたPMMA膜とAN69ST膜のCytokine除去比較
  • 第26回日本急性血液浄化学会学術集会Best Presentation Award(BPA)受賞論文 ブタ敗血症modelを用いたPMMA膜とAN69ST膜のCytokine除去比較
  • ダイ26カイ ニホン キュウセイ ケツエキジョウカガッカイ ガクジュツ シュウカイ Best Presentation Award(BPA)ジュショウ ロンブン ブタ ハイケツショウ model オ モチイタ PMMAマク ト AN69STマク ノ Cytokine ジョキョ ヒカク

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<p>【Introduction】 The efficacy of cytokine elimination during continuous hemofiltration (CHF) largely depends on the characteristics of the membrane filter. AN69ST and polymethyl methacrylate (PMMA) membranes have strong adsorption capacities. We determined the elimination of cytokines by the AN69ST and PMMA membrane filters during CHF in the pig sepsis model. 【Methods】 Piglets weighing 17±3kg (n=7) were anesthetized and endotoxin 30μg/kg was administered. The Baxter sepXiris (AN69ST membrane) and the Toray Hemofeel 1.8W (PMMA membrane) were used as hemofilters. Samples were obtained at 1, 2, 4, and 6hr after beginning endotoxin administration.The inlet plasma, outlet plasma, and filtrate concentrations of TNF-α, IL-1β, IL-6, and IL-8 were measured and the clearance of each cytokine was calculated. 【Results】 Endotoxin administration increased the inlet plasma concentrations of all the measured cytokines. The AN69ST membrane filter showed higher plasma clearance of IL-8 (23.66±17.01mL/min) than PMMA membrane filter (−6.62±48.89mL/min) at 6 hr after beginning endotoxin administration (p＜0.05). However, the PMMA membrane filter showed higher adsorption clearance of IL-1β and IL-6 than the AN69ST membrane filter (p＜0.05). IL-6 did not appear in the filtrate of the PMMA membrane filter, and IL-8 was not eliminated in the filtrate of the AN69ST membrane filter. The filtrate concentration of TNF-α increased after its plasma concentration decreased with the PMMA membrane filter. 【Conclusions】 These results show the differences in adsorption of cytokines by the AN69ST and PMMA membrane filters.</p>

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