Bone-forming ability and anti-inflammatory effect of the nutritional functional food “Osteo AG” in human osteoblastic osteosarcoma cells (Saos-2)
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- OH HOUREI
- Department of Innovation in Dental Education, Osaka Dental University
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- MAKITA YOSHIMASA
- Department of Chemistry, Osaka Dental University
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- OKUSA NOBUTAKA
- Department of Forensic Dentistry, Osaka Dental University
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- MASUNO KAZUYA
- Department of Innovation in Dental Education, Osaka Dental University
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- IMAMURA YASUHIRO
- Department of Pharmacology, Matsumoto Dental University
Bibliographic Information
- Other Title
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- 栄養機能性食品“OsteoAG”によるヒト骨芽細胞性細胞(Saos-2)への骨形成能と抗炎症作用の研究
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Abstract
<p>This study examines the cell proliferation and collagen-producing abilities, alkaline phosphatase (ALP) activity, and the production interleukin (IL)-6 and IL-8 of the nutritional functional food “Osteo AG” in a basic experimental system using Saos-2, a human osteoblastic osteosarcoma cell line. Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay;DNA synthesis by bromodeoxyuridine-incorporation. Production of collagen, IL-6, and IL-8 was analyzed by ELISA while ALP activity by a commercial assay kit. We found that cell proliferation, production of collagen type Ⅰ, and ALP activity increased in the presence of Osteo AG. Although lipopolysaccharide of the periodontopathic bacterium Aggregatibacter actinomycetemcomitans induced production of the aforementioned inflammatory cytokines, Osteo AG inhibited this effect. In conclusion, our study suggests that Osteo AG has bone-forming ability and anti-inflammatory effects in Saos-2. </p>
Journal
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- ORAL THERAPEUTICS AND PHARMACOLOGY
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ORAL THERAPEUTICS AND PHARMACOLOGY 40 (3), 65-71, 2021
JAPANESE SOCIETY OF ORAL THERAPEUTICS AND PHARMACOLOGY
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Keywords
Details 詳細情報について
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- CRID
- 1390292007976578944
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- ISSN
- 18844928
- 02881012
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- Text Lang
- en
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- Data Source
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- JaLC
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- Abstract License Flag
- Disallowed