Development of Capillary Devices for Digital Molecular Sieving Electrophoresis

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  • SUEYOSHI Kenji
    Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University PRESTO, Japan Science and Technology Agency
  • MATSUDA Keita
    Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University
  • ENDO Tatsuro
    Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University
  • HISAMOTO Hideaki
    Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University

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Other Title
  • デジタル分子ふるい電気泳動用キャピラリーデバイスの開発
  • デジタル ブンシ フルイ デンキ エイドウヨウ キャピラリーデバイス ノ カイハツ

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<p>Recently, digital electrophoresis has attracted attention as a separation and preconcentration method based on the passage or non-passage of sample molecules through various functional hydrogels. However, the complex fabrication of the device filled with various hydrogels has been a major issue. In this study, an easy-to-fabricate capillary device for digital electrophoresis is proposed and developed by the successive introduction of prepolymer solutions. Digital molecular sieving electrophoresis using polyacrylamide hydrogels with different densities was employed for a fundamental characterization of the developed device. As a result, the correlation between the total concentration of acrylamide species in the prepolymer solution (%T) and the molecular weights of the passing or non-passing proteins was clarified. The evaluation of the concentration efficiency showed that the sensitivity increased up to about 180 times. In addition, digital molecular sieve electrophoresis using the device successively plugged with 5, 15, and 25 %T polyacrylamide hydrogels achieved simultaneous separation and preconcentration of proteins with different molecular weights. These results demonstrate high-resolution with high-sensitivity digital electrophoresis using the simply fabricated device.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 71 (6), 325-331, 2022-06-05

    The Japan Society for Analytical Chemistry

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