Evaluation of liquid-less biofilm staining on wound blotting samples

DOI
  • Minematsu Takeo
    Department of Skincare Science, Graduate School of Medicine, The University of Tokyo Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo
  • Tomida Sanai
    Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo
  • Tobe Hiromi
    Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo
  • Koudounas Sofoklis
    Department of Skincare Science, Graduate School of Medicine, The University of Tokyo Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo
  • Nakagami Gojiro
    Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo
  • Sanada Hiromi
    Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo

Bibliographic Information

Other Title
  • ウンドブロッティングサンプルのリキッドレス・バイオフォルム染色法の評価

Search this article

Description

Because biofilm infection is a major cause of hard-to-heal wounds, biofilm detection is essential for prevention and management of hard-to-heal wounds. We newly developed a simple, rapid, and safe staining method of biofilms, liquid-less staining. In this study, we evaluated the liquid-less staining in comparison with the conventional method. We prepared dot blot samples of different concentration of artificial biofilms, and stained with the conventional and liquid-less staining. The liquid-less method detected the signals of lower concentration of biofilms than the conventional method in subjective evaluation. Although the quantitative evaluation showed similar signal-noise ratio and correlation between signal intensity and the concentration of biofilms, the liquid-less method was superior to the conventional method in signal stability. The liquid-less staining method is expected to be applied for the point-of-care biofilm testing in home care settings.

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top