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Evaluation of liquid-less biofilm staining on wound blotting samples
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- Minematsu Takeo
- Department of Skincare Science, Graduate School of Medicine, The University of Tokyo Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo
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- Tomida Sanai
- Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo
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- Tobe Hiromi
- Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo
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- Koudounas Sofoklis
- Department of Skincare Science, Graduate School of Medicine, The University of Tokyo Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo
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- Nakagami Gojiro
- Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo
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- Sanada Hiromi
- Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo
Bibliographic Information
- Other Title
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- ウンドブロッティングサンプルのリキッドレス・バイオフォルム染色法の評価
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Description
Because biofilm infection is a major cause of hard-to-heal wounds, biofilm detection is essential for prevention and management of hard-to-heal wounds. We newly developed a simple, rapid, and safe staining method of biofilms, liquid-less staining. In this study, we evaluated the liquid-less staining in comparison with the conventional method. We prepared dot blot samples of different concentration of artificial biofilms, and stained with the conventional and liquid-less staining. The liquid-less method detected the signals of lower concentration of biofilms than the conventional method in subjective evaluation. Although the quantitative evaluation showed similar signal-noise ratio and correlation between signal intensity and the concentration of biofilms, the liquid-less method was superior to the conventional method in signal stability. The liquid-less staining method is expected to be applied for the point-of-care biofilm testing in home care settings.
Journal
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- Journal of Nursing Science and Engineering
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Journal of Nursing Science and Engineering 9 (0), 222-228, 2022
The Society for Nursing Science and Engineering
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Details 詳細情報について
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- CRID
- 1390292936928238464
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- ISSN
- 24326283
- 21884323
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- Text Lang
- en
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- Data Source
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- JaLC
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- Abstract License Flag
- Disallowed