Screening of Antibacterial Efficacy of Chitosan Encapsulated Probiotics (<i>Lactococcus lactis</i> and <i>Lactobacillus curvattus</i>) against Clinical Bacterial Pathogens

  • Nasreen Sundas
    Microbial Biotechnology Laboratory, Department of Zoology, The University of Azad Jammu and Kashmir, King Abdullah Campus
  • Andleeb Saiqa
    Microbial Biotechnology Laboratory, Department of Zoology, The University of Azad Jammu and Kashmir, King Abdullah Campus
  • Ali Shaukat
    Department of Zoology, Government College University
  • Imdad Kaleem
    Department of Bioscience, COMSATS University
  • Awan Uzma Azeem
    Department of Biological Sciences, National University of Medical Sciences (NUMS)
  • Raja Sadaf Azad
    Department of Bioscience, COMSATS University
  • Mughal Tafail Akbar
    Department of Zoology, Women University of Azad Jammu and Kashmir
  • Abbasi Sohail Akbar
    Department of Biotechnology, The University of Azad Jammu and Kashmir

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  • Screening of Antibacterial Efficacy of Chitosan Encapsulated Probiotics (Lactococcus lactis and Lactobacillus curvattus) against Clinical Bacterial Pathogens

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<p>Probiotics frontier in depressing the clinical bacterial pathogens to avoid multidrug resistance phenomenon. The present study aimed to determine the antibacterial efficiency of chitosan encapsulated probiotics isolated from buffalo milk samples against clinical bacterial pathogens. The Agar well method was used for antibacterial activity. Lactococcus lactis (A) and Lactobacillus curvattus (B) were isolated from fresh buffalo milk samples, identified via culturing media, Gram’s staining, biochemical tests, and antibiogram analysis. Encapsulation of probiotics was carried out using chitosan and was characterized via a scanning electron microscope. Antibiogram analysis elicit that L. lactis culture (A1) was highly sensitive to chloramphenicol (17.66±0.47 mm), tobramycin (15.33±0.47 mm), and ciprofloxacin (12.33±0.47 mm) and resistant against tetracycline, Penicillin G, Erythromycin, Amoxycillin, Ceftriaxone, Cephalothin, and Cephradine, while L. curvattus culture (B1) was affected by Ceftriaxone (18.67±0.47 mm), Amoxycillin (14.33±0.94 mm), Cephalothin (13.67±0.47 mm), Erythromycin (13.33±0.47 mm), Penicillin G (12.67±0.47 mm), Cephradine (10.33±0.47 mm), and Chloramphenicol (9.67±0.47 mm) and resistant against tetracycline, Tobramycin, and Ciprofloxacin. Antibacterial efficacy of non-encapsulated probiotic cultures was significant and maximum inhibition of bacterial were recorded compared to their cellular components. SEM of encapsulated probiotics revealed that they were successfully covered with a chitosan protective layer and could be effective as bio-preservatives due to being slowly released at the target site. The current study concluded that L. lactis, L. curvattus, and their cellular components have a significant bactericidal effect against infectious pathogens and could be used as a potential therapeutic drug against infectious diseases.</p>

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