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Screening of Antibacterial Efficacy of Chitosan Encapsulated Probiotics (<i>Lactococcus lactis</i> and <i>Lactobacillus curvattus</i>) against Clinical Bacterial Pathogens
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- Nasreen Sundas
- Microbial Biotechnology Laboratory, Department of Zoology, The University of Azad Jammu and Kashmir, King Abdullah Campus
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- Andleeb Saiqa
- Microbial Biotechnology Laboratory, Department of Zoology, The University of Azad Jammu and Kashmir, King Abdullah Campus
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- Ali Shaukat
- Department of Zoology, Government College University
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- Imdad Kaleem
- Department of Bioscience, COMSATS University
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- Awan Uzma Azeem
- Department of Biological Sciences, National University of Medical Sciences (NUMS)
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- Raja Sadaf Azad
- Department of Bioscience, COMSATS University
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- Mughal Tafail Akbar
- Department of Zoology, Women University of Azad Jammu and Kashmir
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- Abbasi Sohail Akbar
- Department of Biotechnology, The University of Azad Jammu and Kashmir
Bibliographic Information
- Other Title
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- Screening of Antibacterial Efficacy of Chitosan Encapsulated Probiotics (Lactococcus lactis and Lactobacillus curvattus) against Clinical Bacterial Pathogens
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Description
<p>Probiotics frontier in depressing the clinical bacterial pathogens to avoid multidrug resistance phenomenon. The present study aimed to determine the antibacterial efficiency of chitosan encapsulated probiotics isolated from buffalo milk samples against clinical bacterial pathogens. The Agar well method was used for antibacterial activity. Lactococcus lactis (A) and Lactobacillus curvattus (B) were isolated from fresh buffalo milk samples, identified via culturing media, Gram’s staining, biochemical tests, and antibiogram analysis. Encapsulation of probiotics was carried out using chitosan and was characterized via a scanning electron microscope. Antibiogram analysis elicit that L. lactis culture (A1) was highly sensitive to chloramphenicol (17.66±0.47 mm), tobramycin (15.33±0.47 mm), and ciprofloxacin (12.33±0.47 mm) and resistant against tetracycline, Penicillin G, Erythromycin, Amoxycillin, Ceftriaxone, Cephalothin, and Cephradine, while L. curvattus culture (B1) was affected by Ceftriaxone (18.67±0.47 mm), Amoxycillin (14.33±0.94 mm), Cephalothin (13.67±0.47 mm), Erythromycin (13.33±0.47 mm), Penicillin G (12.67±0.47 mm), Cephradine (10.33±0.47 mm), and Chloramphenicol (9.67±0.47 mm) and resistant against tetracycline, Tobramycin, and Ciprofloxacin. Antibacterial efficacy of non-encapsulated probiotic cultures was significant and maximum inhibition of bacterial were recorded compared to their cellular components. SEM of encapsulated probiotics revealed that they were successfully covered with a chitosan protective layer and could be effective as bio-preservatives due to being slowly released at the target site. The current study concluded that L. lactis, L. curvattus, and their cellular components have a significant bactericidal effect against infectious pathogens and could be used as a potential therapeutic drug against infectious diseases.</p>
Journal
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- Journal of Oleo Science
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Journal of Oleo Science 71 (9), 1363-1374, 2022
Japan Oil Chemists' Society