Determination of Chlorothalonil Metabolite I in Livestock Products by LC-MS/MS
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- Kobayashi Maki
- Tokyo Metropolitan Institute of Public Health
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- Sakai Naoko
- Tokyo Metropolitan Institute of Public Health
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- Ohmachi Yuki
- Tokyo Metropolitan Institute of Public Health
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- Morita Yuka
- Tokyo Metropolitan Institute of Public Health
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- Nemoto Satoru
- 国立医薬品食品衛生研究所食品添加物指定等相談センターFood Additive Designation Consultation Center, National Institute of Health Sciences
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- Ohtsuka Kenji
- Tokyo Metropolitan Institute of Public Health
Bibliographic Information
- Other Title
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- LC-MS/MSによる畜産物中のクロロタロニル代謝物I分析法
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Abstract
<p>An analytical method based on LC-MS/MS was developed for the determination of chlorothalonil metabolite I in livestock products. Chlorothalonil metabolite I in livestock products was extracted with acetone. The crude extracts were defatted by acetonitrile and n-hexane partitioning. Cleanup was carried out using a combination of ethylene diamine-N-propyl silylation silica gel (PSA) and silica gel (SI) mini columns with acidic condition. The sample solution was subjected to LC-MS/MS using an external solvent calibration curve. The average recovery (n=5) of chlorothalonil metabolite I from five types of livestock products (cattle muscle, cattle fat, cattle liver, milk and egg) spike at the maximum residue limits (MRLs) or at a uniform limit of 0.01 mg/kg was 97.1–102.9%, with a relative standard deviation of 1.4–6.8%. The limit of quantitation of the developed method was calculated to be 0.01 mg/kg</p>
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 63 (6), 195-201, 2022-12-25
Japanese Society for Food Hygiene and Safety