Examples and Prospects of Electron 3D Crystallography/3D ED/microED

Yonekura Koji, Maki-Yonekura Saori, Takaba Kiyofumi

doi:10.11410/kenbikyo.57.3_118

<p>Electron 3D crystallography, which is now considered as one of the major cryo-EM modalities, can determine 3D structures from undersized crystals. It can even reveal sub-atomic resolution structures of various samples including organic compounds, which are not compatible with single particle cryo-EM and cryo-electron tomography. Thus, its application is not limited to life science but extended to synthetic organic chemistry, pharmaceutical and material sciences, and related areas. We have been involved in development of this technique since its early stage, and now rapid structure determination is routinely done by AI data collection and automated data processing from small crystals in organic solvent, water solution, and powders. This review covers the background of this technique and our recent results on a new double helix structure built by self-assembly of nanographene, thin crystals of an organic semiconductor, and fibrous crystals of polypeptides related to a neurological disease, amyotrophic lateral sclerosis (ALS). The structure of the organic semiconductor includes disorder regions, which may be useful for design of new soft devices. The polypeptide structures of the wild-type and a toxic mutant may suggest how amyloid fibers aggregate. We also discuss the limitation and merits of electron diffraction in comparison with XFEL crystallography.</p>

Examples and Prospects of Electron 3D Crystallography/3D ED/microED

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