Insights into Antifungal Mechanisms of <i>Bacillus velezensis</i> S141 against <i>Cercospora</i> Leaf Spot in Mungbean (<i>V. radiata</i>)
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- Songwattana Pongpan
- School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
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- Boonchuen Pakpoom
- School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
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- Piromyou Pongdet
- School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
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- Wongdee Jenjira
- School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
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- Greetatorn Teerana
- School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
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- Inthaisong Sukanya
- School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology
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- Alisha Tantasawat Piyada
- School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology
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- Teamtisong Kamonluck
- The Center for Scientific and Technological Equipment, Suranaree University of Technology
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- Tittabutr Panlada
- School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
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- Boonkerd Nantakorn
- School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
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- Teaumroong Neung
- School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Bibliographic Information
- Other Title
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- Insights into Antifungal Mechanisms of Bacillus velezensis S141 against Cercospora Leaf Spot in Mungbean (V. radiata)
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Description
<p>Cercospora leaf spot (CLS) is caused by Cercospora canescens and is one of the most important diseases of mungbean (Vigna radiata). Cercospora leaf spot may result in economic loss in production areas. The present study investigated the potential of Bacillus velezensis S141 as a biocontrol agent for C. canescens PAK1 growth on culture plates. Cell-free secretions from a dual culture of S141+PAK1 inhibited fungal growth more than those from a single culture of S141. The biocontrol efficiency of S141 against Cercospora leaf spot on mungbean was then evaluated by spraying. The disease severity of Cercospora leaf spot was significantly reduced in plants treated with S141, with a control efficiency of 83% after 2 days of infection. Comparative transcriptomics and qRT-PCR analyses of S141 during C. canescens inhibition were performed to elucidate the antifungal mechanisms underlying its antifungal activity against Cercospora leaf spot. According to the differentially expressed genes, most up-regulated genes involved in the biosynthetic genes encoding enzymatic hydrolases, including protease, β-glucanase, and N-acyl glucosaminase, were detected in strain S141 following its interaction. Moreover, genes related to secondary metabolites (surfactin, bacilysin, and bacillomycin D) were up-regulated. Collectively, these results suggest that S141 exhibited strong antifungal activity against C. canescens due to multiple enzymatic hydrolases and secondary metabolites. Therefore, the present study provides insights into the biological network responsible for the antifungal activity of B. velezensis S141 against C. canescens.</p>
Journal
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- Microbes and Environments
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Microbes and Environments 38 (1), n/a-, 2023
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
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Details 詳細情報について
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- CRID
- 1390295491685608448
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- NII Book ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL BIB ID
- 032835199
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- PubMed
- 36935122
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
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- Abstract License Flag
- Disallowed