Molecular Epidemiology of Penicillinase-Producing Neisseria gonorrhoeae Isolates and Their bla[TEM-135] Gene Variant in Bangkok, Thailand, 2015-2017
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- Nokchan Natakorn
- Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand
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- Nitayanon Perapon
- Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand
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- Tribuddharat Chanwit
- Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand
書誌事項
- タイトル別名
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- Molecular Epidemiology of Penicillinase-Producing <i>Neisseria gonorrhoeae</i> Isolates and Their <i>bla</i><sub>TEM-135</sub> Gene Variant in Bangkok, Thailand, 2015–2017
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<p>Penicillinase-producing Neisseria gonorrhoeae (PPNG) possessing blaTEM-135 is a serious public health threat. With only a single change in the amino acid sequence, blaTEM-135 could evolve into a TEM-type extended-spectrum beta-lactamase (ESBL), which hydrolyzes extended-spectrum cephalosporins, including ceftriaxone and cefixime. We investigated the molecular epidemiological characteristics, types of plasmids in PPNG isolates, and prevalence of PPNG clinical isolates producing TEM-135 beta-lactamases. N. gonorrhoeae multi-antigen sequence typing (NG-MAST) was used to determine the molecular epidemiological characteristics of 99 PPNG isolates collected from 2015 to 2017. A mismatch amplification mutation assay was used to examine the blaTEM-135 gene prevalence. Of the 89 identified NG-MAST sequence types, 65 (73.0%) were novel. Only 17.7% (43/243) of PPNG isolates belonged to 16 genogroups. The most frequent plasmid was African, followed by Rio/Toronto, and Asian. The blaTEM-135 allele was found in Rio/Toronto plasmids. The blaTEM-135 allele was present in 23.2% (23/99) of the PPNG isolates. PPNG isolates expressing TEM-135 beta-lactamase exhibited significantly higher penicillin MIC (minimum inhibitory concentration) values than TEM-1 PPNG isolates. The PPNG isolates showed high genetic diversity and a high proportion of blaTEM-135 alleles. Mutation of the blaTEM-135 allele is worrisome as only one mutation could cause TEM-1 to evolve into an ESBL variant that degrades ceftriaxone. Ongoing surveillance of blaTEM-135 and new PPNG isolates is imperative.</p>
収録刊行物
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 76 (2), 126-134, 2023-03-31
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詳細情報 詳細情報について
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- CRID
- 1390295524492376448
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- NII書誌ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
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- NDL書誌ID
- 032759521
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- PubMed
- 36450575
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
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- 抄録ライセンスフラグ
- 使用不可