Survival of anaerobic fungus <i>Caecomyces</i> sp. in various preservation methods: a comparative study

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  • Nagpal Ravinder
    Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, Haryana, India
  • Puniya Anil Kumar
    Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, Haryana, India
  • Sehgal Jatinder Paul
    Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal 132001, Haryana, India
  • Singh Kishan
    Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, Haryana, India

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  • Survival of anaerobic fungus Caecomyces sp. in various preservation methods : a comparative study

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<p>The present investigation was designed to observe the survival of the anaerobic fungus Caecomyces sp. in various routine preservation methods. Among all the treatments, cryopreservation of fungi at −70°C with glycerol was found to be most effective for long-term maintenance (more than 90 days) of rumen fungi, followed by dimethyl sulfoxide (DMSO) and ethylene glycol (up to 60 days). In contrast, at −196°C, DMSO showed maximum survival (more than 90 days), followed by glycerol (up to 90 days) and ethylene glycol (up to 30 days). At 39°C, maximum survival (up to 30 days) was observed with soft agar and wheat straw; at refrigeration temperature, preservation with Orpin's media containing straw showed maximum survival (up to 30 days).</p>

Journal

  • Mycoscience

    Mycoscience 53 (6), 427-432, 2012

    The Mycological Society of Japan

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