MicroRNA Extraction from Extracellular Vesicles in Body Fluids Using PMMA-based Nanowire Devices
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- SHIMADA Taisuke
- Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University
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- TAKESHITA Daiki
- Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University
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- ITO Satoru
- Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University
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- YASUI Takao
- Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University Japan Science and Technology Agency (JST), PRESTO
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- BABA Yoshinobu
- Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University Institute of Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology
Bibliographic Information
- Other Title
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- PMMA製ナノワイヤデバイスによる体液中細胞外小胞由来microRNA抽出
- PMMAセイ ナノワイヤデバイス ニ ヨル タイエキ チュウ サイボウ ガイ ショウホウユライ microRNA チュウシュツ
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Abstract
<p>MicroRNAs (miRNAs) within extracellular vesicles (EVs) in body fluids may serve as biomarkers for low-invasive early diseases diagnostics. Although isolation of EVs in body fluids is a crucial step for analyzing miRNAs within the EVs, conventional techniques face challenges due to their low isolation efficiency and requirements of large sample volume. In this work, we propose a method for efficient EV isolation and in-situ miRNA extractions using a disposable microfluidic device with ZnO nanowires. The device has a microchannel on a polymer substrate and the ZnO nanowires are embedded inside the microchannel. The device allows for capturing EVs on nanowire surfaces from biological fluids and extracting miRNAs based on chemical lysis of the captured EVs. Two important factors for the efficient capture mechanism are large surface area of the nanowires and electrostatic interactions between the nanowires and EVs. Our demonstrations confirmed the device isolated EVs from 1 mL of cell supernatant and serum more efficiently in comparison to conventional ultracentrifugation and polymeric precipitation methods. Moreover, by using microarray-based detections of miRNAs extracted from the EVs in human serum, the device also presented a larger number of the detected miRNA types than the conventional methods. EV isolation and miRNA extractions using the device may contribute to realize low-invasive early disease diagnosis and new biomarker development.</p>
Journal
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- BUNSEKI KAGAKU
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BUNSEKI KAGAKU 72 (3), 105-110, 2023-03-05
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390295669572739584
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- NII Book ID
- AN00222633
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- NDL BIB ID
- 032957026
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- ISSN
- 05251931
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
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- Abstract License Flag
- Disallowed