A Study of Submandibular Gland Changes in Mice of a Murine Model of Sjogren's Syndrome Administered Dental Pulp Stem Cell-Conditioned Medium
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- Usui Seiko
- Department of Oral and Maxillofacial Surgery, The Nippon Dental University School of Life Dentistry at Niigata
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- Takahashi Haruka
- Department of Oral and Maxillofacial Surgery, The Nippon Dental University School of Life Dentistry at Niigata Division of Cell Regeneration and Transplantation, Advanced Research Center School of Life Dentistry at Niigata, The Nippon Dental University Niigata
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- Tanaka Akira
- Department of Oral and Maxillofacial Surgery, The Nippon Dental University School of Life Dentistry at Niigata Division of Cell Regeneration and Transplantation, Advanced Research Center School of Life Dentistry at Niigata, The Nippon Dental University Niigata
書誌事項
- タイトル別名
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- A Study of Submandibular Gland Changes in Mice of a Murine Model of Sjögren’s Syndrome Administered Dental Pulp Stem Cell-Conditioned Medium
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説明
<p>Recently researchers are attempting to develop a treatment for Sjögren’s syndrome using mesenchymal stem cell culture supernatant. In this study, we analyzed changes in the submandibular gland in Murphy Roths Large (MRL) mice of a Sjögren’s syndrome model administered human dental pulp stem cell-derived conditioned medium (DPSC-CM). MRL mice were injected with DPSC-CM twice per week for two weeks. Submandibular glands were analyzed after four days of final injection in after 4 days group and 29 days after final injection in after 29 days group, which were compared to each non-treated group. We measured salivary flow and conducted general histological, immunohistochemical, and quantitative real-time polymerase chain reaction (PCR) investigations. Both the after 4 and 29 days groups of mice treated with DPSC-CM showed significant increase in salivary flow compared to the non-treated group. Significant increases were observed in the stem cell marker (c-Kit), progenitor cell marker (CK5), and acinar cell marker (AQP5) in immunohistochemical analyses. Furthermore, a significant increase in the mRNA expression levels of anti-inflammatory cytokines TGF-β1 and IL-10 was observed. There was a significant decrease in the inflammatory cytokine IL-6. The results of this study show that the systemic administration of DPSC-CM to MRL mice restores the function of submandibular gland tissue and reduces inflammation. Furthermore, because the effects were observed even after 29 days of DPSC-CM administration, it was suggested that there would be long-term functional repair of submandibular gland tissue and control of inflammation. DPSC-CM showed a potential as long-term treatment for hyposalivation caused by Sjögren’s syndrome.</p>
収録刊行物
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 32 (2), 119-126, 2023
硬組織再生生物学会
