Analysis of Acetylated Hyaluronic Acid in Moisturizing Lotion and Milk Lotion by HPLC with Fluorescence Detection
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- Shimekake Momo
- Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- Komeno Masahiro
- Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- Taguchi Manawo
- Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- Katsumoto Shoo
- Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- Tanda Yuna
- Graduate School of Pharmaceutical Sciences, Chiba University
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- Sato Kazuki
- Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- Wada Takeshi
- Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- Toida Toshihiko
- Graduate School of Pharmaceutical Sciences, Chiba University
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- Higashi Kyohei
- Faculty of Pharmaceutical Sciences, Tokyo University of Science
抄録
<p>We developed a simple and sensitive analytical HPLC method for the determination of acetylated hyaluronic acid (AcHA) in moisturizing and milk lotions. AcHA with different molecular weights was separated as a single peak using a C4 column and detected through post-column derivatization using 2-cyanoacetamide. The limits of detection and quantification were 60 and 200 ng, respectively. We found that AcHA in water was successfully extracted into a strong anion exchange (SAX) spin column with a recovery rate of AcHA was 63.8 ± 1.8%. Although the supernatant from acetone precipitation of lotions could pass through the spin column, the recovery rate (%) and accuracy of AcHA were affected by the viscous properties of cosmetics and acidic and acetone-soluble ingredients. Upon conducting analytical methods in this study, the concentration of AcHA in nine lotions was found to have ranged from 7.50 to 83.3 µg/mL. These values are comparable to the concentration range of AcHA in emulsions that have been previously evaluated for their superior effects. We believe that the analytical and extraction method is useful for the qualitative analysis of AcHA in moisturizing and milk lotions.</p>
収録刊行物
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- ＣＨＥＭＩＣＡＬ ＆ ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ ＢＵＬＬＥＴＩＮ
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ＣＨＥＭＩＣＡＬ ＆ ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ ＢＵＬＬＥＴＩＮ 71 (7), 616-619, 2023-07-01
公益社団法人 日本薬学会