HPLC Separation of Acetylsalicylic Acid and Its Related Compounds with Core-shell Type Reversed-phase Columns and Rapid Evaluation of Stability of Aspirin Tablets Suspension

DOI Web Site Web Site 8 References
  • MURAOKA Haruka
    Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Yasuda Women’s University
  • SUMIDA Nana
    Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Yasuda Women’s University
  • MORITA Shihomi
    Hiroshima Red Cross Hospital & Atomic-bomb Survious Hospital
  • KAWABATA Kohei
    Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Yasuda Women’s University
  • NISHI Hiroyuki
    Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Yasuda Women’s University

Bibliographic Information

Other Title
  • コアシェル型逆相充てん剤カラムによるアセチルサリチル酸類のHPLC分離とアスピリン懸濁製剤の迅速な安定性評価
  • コアシェルガタ ギャクソウ ジュウテンザイ カラム ニ ヨル アセチルサリチル サンルイ ノ HPLC ブンリ ト アスピリン ケンダク セイザイ ノ ジンソク ナ アンテイセイ ヒョウカ

Search this article

Abstract

<p>This paper describes the separation and determination of acetylsalicylic acid (aspirin, AP) and its related compounds by HPLC employing various core-shell (CS) type reversed-phase columns. Among seven CS type columns, large retention was observed in a pentabromobenzyl (PBr) type column, on the other hand, cyanopropyl (CN) type column gave low retention. For the elution order of eleven compounds investigated, caffeine retained in a PBr column and a Biphenyl column compared with those in other columns, showing effective π–π interaction. Retention order of acetylsalicylic acid and its decomposed compound salicylic acid (o-SA) was reversed in a RP-Amide column, showing effective electrostatic interaction between the column and the analyte. From the investigation of each CS column selectivity, a Biphenyl column was selected for the fast assay of Aspirin tablets due to the largest resolution between AP and o-SA. Under the HPLC conditions of a mobile phase, acetonitrile 30 % and 0.05 mol L−1 phosphate buffer (pH 3.0), a Biphenyl column, and ethenzamide as an internal substance (IS), a fast determination of AP in tablets by an IS method was successfully achieved within 2.5 min. Stability of AP changed dosage form (suspension in water) and AP suspension mixed with other suspended medicine Magmitt (MgO) was evaluated by the method. After storage for 30 min at room temperature, around 20 % AP was decomposed to o-SA in the mixed suspension medicine, indicating the loss of AP effectiveness (platelet aggregation inhibitory activity).</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 72 (7.8), 299-305, 2023-07-05

    The Japan Society for Analytical Chemistry

References(8)*help

See more

Keywords

Report a problem

Back to top