Construction of Arabidopsis Transformants for Live Cell-imaging of Meiotic Chromosomes and Analysis of their Dynamics

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  • 減数分裂期染色体ライブ観察用のシロイヌナズナ形質転換体の作成とその染色体の動態解析
  • ゲンスウ ブンレツキ センショクタイ ライブ カンサツヨウ ノ シロイヌナズナ ケイシツ テンカンタイ ノ サクセイ ト ソノ センショクタイ ノ ドウタイ カイセキ

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Previously, we developed a line of Arabidopsis thaliana transformant for live cellimaging of meiotic chromosomes by introducing the fusion gene of the histone H2B gene and GFP gene under the control of the AtDMC1 promoter (DHG). Using a transformant (DHG1 line), we were able to observe meiotic chromosomes in living pollen mother cells (PMCs). In the DHG1 line, the fusion gene was transferred into chromosome 3, and DHG expression was not specific to PMCs, but also observed in also other cells constituting its anther. We need more DHG lines, which are transferred DHG gene into chromosomes other than chromosome 3, and show more PMC-specific expression. We introduced DHG into A . thaliana again, and obtained new DHG lines as survivors on selective media. Some lines were actually confirmed to be transformants by PCR analysis for the GFP gene. Three lines were further examined using confocal scanning laser microscopy, and meiotic chromosomes in several stages were clearly observed in their PMCs.

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