Development of a Cartridge Device Integrated with Multiple Hydrogels Exhibiting Different Functionalities for Two-dimensional Digital Electrophoresis

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  • KANAOKA Tadamasa
    Department of Material Chemistry, Graduate School of Engineering, Osaka Prefecture University
  • ENDO Tatsuro
    Department of Material Chemistry, Graduate School of Engineering, Osaka Metropolitan University
  • HISAMOTO Hideaki
    Department of Material Chemistry, Graduate School of Engineering, Osaka Metropolitan University
  • SUEYOSHI Kenji
    Department of Material Chemistry, Graduate School of Engineering, Osaka Metropolitan University CREST, Japan Science and Technology Agency

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Other Title
  • 二次元デジタル電気泳動分離のための異種機能性ハイドロゲル集積カートリッジデバイスの開発
  • ニジゲン デジタル デンキ エイドウ ブンリ ノ タメ ノ イシュ キノウセイ ハイドロゲル シュウセキ カートリッジデバイス ノ カイハツ

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Abstract

<p>A two-dimensional electrophoresis method, involving involves isoelectric focusing in the first dimension and polyacrylamide gel electrophoresis in the second dimension, is commonly used for the separation and analysis of protein mixtures. However, conventional methods have some drawbacks such as complex experimental procedures, long analysis time, and high consumption of reagents. In this study, based on the knowledge gained from our previous research on digital electrophoresis, a cartridge device with heterogeneous functionalized gels arranged and integrated in a two-dimensional pattern was used. The fundamental evaluation using model proteins confirmed the feasibility of sample introduction using the developed devices, digital sieving electrophoresis, and digital isoelectric focusing using pH-buffered gels. Furthermore, the verification of two-dimensional digital electrophoresis using a cartridge device with multiple pH-buffered gels and molecular sieving gels demonstrated continuous two-dimensional digital separation by employing the single device.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 72 (9), 369-375, 2023-09-05

    The Japan Society for Analytical Chemistry

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