書誌事項
- タイトル別名
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- Analytical method of residual oxytetracycline in apple juices using HPLC
- HPLC オ モチイ タリン ゴジュース チュウ オキシテトラサイクリン ノ ザンリュウ シケンホウ
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説明
An analytical method using HPLC was developed to analyze residual oxytetracycline (OTC) in apple juices. Residual OTC was extracted with a citrate-phosphate buffer containing EDTA-2Na. The matrix was removed from the extract by a solid-phase column, InertSep PLS-2 (265 mg/20 mL). After removing the solvent, the extract was resolved in a potassium phosphate solution. OTC in the test solution was determined quantitatively using HPLC with a photodiode array detector (PDA). The interfering peaks were not observed in chromatograms of blank extracts, indicating the high selectivity of this method. The limit of quantification of this method was 0.01 µg/g in apple juices. Method validation was performed by recovery tests at 0.2 µg/g to evaluate the trueness, repeatability (RSDr), and with-in laboratory reproducibility (RSDwr). The validation results for two types of apple juice exhibited sufficient recovery (99% and 85%) and precision (RSDr ≦ 4% and RSDwr ≦ 5%). The time required for the preparation of eight test solutions was less than three hours. This method can help inspect residual OTC in apple juices.
収録刊行物
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- 日本食品化学学会誌
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日本食品化学学会誌 30 (3), 178-183, 2023-12-25
日本食品化学学会
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詳細情報 詳細情報について
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- CRID
- 1390298588085274368
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- NII書誌ID
- AA11666400
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- ISSN
- 21896445
- 13412094
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- NDL書誌ID
- 033269262
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可