High Concentrations of Nucleotides Prevent Capillary Regression during Hindlimb Unloading by Inhibiting Oxidative Stress and Enhancing Mitochondrial Metabolism of Soleus Muscles in Rats

DOI HANDLE Web Site 参考文献36件 オープンアクセス
  • Nakanishi Ryosuke
    Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences Department of Physical Therapy, Kobe International University
  • Hashimoto Nagisa
    Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences
  • Takuwa Miho
    Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences
  • Xing Jihao
    Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences
  • Uemura Mikiko
    Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences Department of Physical Therapy, Kansai University of Welfare Sciences
  • un Nisa Badur
    Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences
  • Tanaka Masayuki
    Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences Department of Physical Therapy, Okayama Healthcare Professional University
  • Hirabayashi Takumi
    Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences
  • Tanaka Minoru
    Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences Department of Rehabilitation, Osaka Health Science University
  • Fujino Hidemi
    Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences

抄録

<p>Prolonged inactivity in skeletal muscles decreases muscle capillary development because of an imbalance between pro- and antiangiogenic signals, mitochondrial metabolism disorders, and increased oxidative stress. Nucleotides have been shown to exert a dose-dependent effect on disuse-induced muscle atrophy. However, the dose-dependent effect on capillary regression in disused muscles remains unclear. Therefore, this study investigated the dose-dependent effect of nucleotides on capillary regression due to disuse. For this purpose, Wistar rats were divided into five groups as follows: control rats fed nucleotide-free diets (CON), hindlimb-unloaded rats fed nucleotide-free diets (HU), and hindlimb-unloaded rats fed 1.0%, 2.5%, and 5.0% nucleotide diets, (HU + 1.0% NT), (HU + 2.5% NT), and (HU + 5.0% NT), respectively. Unloading increased reactive oxygen species (ROS) production and decreased mitochondrial enzyme activity, thereby decreasing the number of muscle capillaries. In contrast, 5.0% nucleotide-containing diet prevented increases in ROS production and reductions in the expression levels of NAMPT, PGC-1α, and CPT-1b proteins. Moreover, 5.0% nucleotide-containing diet prevented mitochondrial enzyme activity (such as citrate synthase and beta-hydroxy acyl-CoA dehydrogenase activity) via NAMPT or following PGC-1α upregulation, thereby preventing capillary regression. Therefore, 5.0% nucleotide-containing diet is likely to prevent capillary regression by decreasing oxidative stress and increasing mitochondrial metabolism.</p>

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