High Concentrations of Nucleotides Prevent Capillary Regression during Hindlimb Unloading by Inhibiting Oxidative Stress and Enhancing Mitochondrial Metabolism of Soleus Muscles in Rats
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- Nakanishi Ryosuke
- Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences Department of Physical Therapy, Kobe International University
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- Hashimoto Nagisa
- Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences
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- Takuwa Miho
- Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences
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- Xing Jihao
- Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences
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- Uemura Mikiko
- Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences Department of Physical Therapy, Kansai University of Welfare Sciences
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- un Nisa Badur
- Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences
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- Tanaka Masayuki
- Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences Department of Physical Therapy, Okayama Healthcare Professional University
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- Hirabayashi Takumi
- Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences
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- Tanaka Minoru
- Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences Department of Rehabilitation, Osaka Health Science University
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- Fujino Hidemi
- Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences
抄録
<p>Prolonged inactivity in skeletal muscles decreases muscle capillary development because of an imbalance between pro- and antiangiogenic signals, mitochondrial metabolism disorders, and increased oxidative stress. Nucleotides have been shown to exert a dose-dependent effect on disuse-induced muscle atrophy. However, the dose-dependent effect on capillary regression in disused muscles remains unclear. Therefore, this study investigated the dose-dependent effect of nucleotides on capillary regression due to disuse. For this purpose, Wistar rats were divided into five groups as follows: control rats fed nucleotide-free diets (CON), hindlimb-unloaded rats fed nucleotide-free diets (HU), and hindlimb-unloaded rats fed 1.0%, 2.5%, and 5.0% nucleotide diets, (HU + 1.0% NT), (HU + 2.5% NT), and (HU + 5.0% NT), respectively. Unloading increased reactive oxygen species (ROS) production and decreased mitochondrial enzyme activity, thereby decreasing the number of muscle capillaries. In contrast, 5.0% nucleotide-containing diet prevented increases in ROS production and reductions in the expression levels of NAMPT, PGC-1α, and CPT-1b proteins. Moreover, 5.0% nucleotide-containing diet prevented mitochondrial enzyme activity (such as citrate synthase and beta-hydroxy acyl-CoA dehydrogenase activity) via NAMPT or following PGC-1α upregulation, thereby preventing capillary regression. Therefore, 5.0% nucleotide-containing diet is likely to prevent capillary regression by decreasing oxidative stress and increasing mitochondrial metabolism.</p>
収録刊行物
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- Acta Histochemica et Cytochemica
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Acta Histochemica et Cytochemica 56 (6), 95-104, 2023-12-28
日本組織細胞化学会
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詳細情報 詳細情報について
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- CRID
- 1390298610032053248
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- ISSN
- 13475800
- 00445991
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- HANDLE
- 20.500.14094/0100486209
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- Crossref
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- 抄録ライセンスフラグ
- 使用不可