Release of SMP30 in Extracellular Vesicles under Conditions of Ascorbic Acid Deficiency Is Involved with Acute Phase Response in ODS Rat

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  • ARAKAWA Kohta
    Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture
  • INOUE Hirofumi
    Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture
  • ISHIGAMI Akihito
    Molecular Regulation of Aging, Tokyo Metropolitan Institute for Geriatrics and Gerontology
  • SATO Ayami
    Molecular Regulation of Aging, Tokyo Metropolitan Institute for Geriatrics and Gerontology
  • TAKINO Yuka
    Molecular Regulation of Aging, Tokyo Metropolitan Institute for Geriatrics and Gerontology
  • TANAKA Miori
    Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture
  • MORIMOTO Hiromu
    Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture
  • TAKAHASHI Nobuyuki
    Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture
  • UEHARA Mariko
    Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture

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<p>Senescence marker protein-30 (SMP30) is a senescence marker molecule that exhibits lactonase activity in the ascorbic acid (AsA) biosynthesis pathway, except in primate mammals, including humans. Although numerous studies have shown that hepatic AsA deficiency causes acute-phase responses, details of the relationship between SMP30 expression and acute-phase responses in AsA-deficient conditions remain to be elucidated. Here, we investigated the effects of AsA deficiency on the relationship between SMP30 and acute liver injury in osteogenic disorder Shionogi (ODS) rats, which have a hereditary defect in AsA biosynthesis. Male-ODS rats (4 wk old) were pair-fed an AsA-free diet with distilled or 0.1% AsA-dissolved water for 14 d. Under AsA-deficient conditions, hepatic SMP30 protein level was decreased and liver injury markers, the serum aspartate aminotransferase/alanine transaminase ratio and cytokine-induced neutrophil chemoattractant-1 (CINC-1) concentration, were elevated. In contrast, SMP30 protein level in extracellular vesicles (EVs) was significantly increased in addition to the positive acute proteins haptoglobin and asialoglycoprotein receptor 1 (ASGPR1), hepatic-derived specific markers expression under AsA-deficient conditions. AsA deficiency also activated signal transducer and activator of transcription 3 (STAT3) which is linked to EVs release in the liver. These results suggest that the release of SMP30 in EVs by AsA deficiency is involved with acute-phase responses.</p>

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